The influence of physical activity on the profile of immune response cells and cytokine synthesis in mice with experimental breast tumors induced by 7,12-dimethylbenzanthracene
The influence of physical activity on the profile of immune response cells and cytokine synthesis in mice with experimental breast tumors induced by 7,12-dimethylbenzanthracene
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DOI：
10.1097/cej.0b013e3283592cbb
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发表时间：
2013-05-01
影响因子：
2.4
通讯作者：
Michelin, Marcia A.
中科院分区：
文献类型：
--
作者：
Abdalla, Douglas R.;Murta, Eddie F. C.;Michelin, Marcia A.
本研究旨在探讨乳腺肿瘤患者淋巴细胞的细胞因子合成及其与体力活动的相互作用。在这项研究中，我们使用了56只雌性Balb/c，8周龄，处女小鼠，体重在20和30 g之间。将小鼠分为四组：无肿瘤/未训练的对照组;无肿瘤/接受水中游泳的体育训练的训练组（30 +/- 4 ℃）45分钟，每周5次，持续8周;肿瘤/未经训练（久坐）组，其中动物接受7，12-二甲基苯并蒽[（DMBA）每周1 mg/ml，持续6周];以及肿瘤/训练组，其中动物经受上述DMBA肿瘤诱导和游泳训练方案。实验期后，从脾细胞样本中收集免疫细胞，置于培养物中，并用脂多糖刺激。通过流式细胞术和酶联免疫吸附试验评估分化簇（CD）3、CD 4和CD 8标志物的存在以及干扰素-γ、白细胞介素（IL）-2、IL-4、IL-10、IL-12、转化生长因子β和肿瘤坏死因子α细胞因子的表达。体力活动增加了产生干扰素γ、IL-2、IL-12和肿瘤坏死因子α的淋巴细胞的数量，减少了表达IL-4、IL-10和转化生长因子β的淋巴细胞和巨噬细胞的数量。相反，在没有游泳训练的情况下，肿瘤诱导降低了Th 1细胞因子水平，同时增加了Th 2细胞因子和Treg细胞的存在。体力活动促进肿瘤发生率的降低，并促进免疫系统向抗肿瘤Th 1反应模式的极化。欧洲癌症预防杂志22：251-258（C）2013年威科健康垂直酒吧利平科特威廉姆斯&威尔金斯。
This study aims to investigate cytokine synthesis by lymphocytes in the presence of mammary tumors and the interaction with physical activity. For this study, we used 56 female Balb/c, 8-week-old, virgin mice with a body mass between 20 and 30 g. The mice were divided into four groups: a no tumor/nontrained control group; a no tumor/trained group subjected to physical training of swimming in water (30 +/- 4 degrees C) for 45 min, five times per week for 8 weeks; a tumor/nontrained (sedentary) group in which the animals received 7,12-dimethylbenzanthracene [(DMBA) 1 mg/ml weekly for 6 weeks)]; and a tumor/trained group in which animals were subjected to the aforementioned DMBA tumor induction and swim training protocols. After the experimental period, immune cells were collected from spleen cell specimens, placed in culture, and stimulated with lipopolysaccharide. The presence of cluster of differentiation (CD)3, CD4, and CD8 markers and the expression of interferon-gamma, interleukin (IL)-2, IL-4, IL-10, IL-12, transforming growth factor beta, and tumor necrosis factor alpha cytokines were assessed by flow cytometry and enzyme-linked immunosorbent assay. Physical activity increased the quantities of lymphocytes producing interferon gamma, IL-2, IL-12, and tumor necrosis factor alpha and decreased the quantities of lymphocytes and macrophages expressing IL-4, IL-10, and transforming growth factor beta. In contrast, tumor induction, in the absence of swim training, reduced Th1 cytokine levels while increasing the presence of Th2 cytokines and Treg cells. Physical activity promoted reductions in the incidence of tumor development and promoted immune system polarization toward an antitumor Th1 response pattern profile. European Journal of Cancer Prevention 22:251-258 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.