Heterogeneous Expression of CT10, CT45 and GAGE7 Antigens and their Prognostic Significance in Human Breast Carcinoma
Heterogeneous Expression of CT10, CT45 and GAGE7 Antigens and their Prognostic Significance in Human Breast Carcinoma
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CT10、CT45 和 GAGE7 抗原在人乳腺癌中的异质表达及其预后意义
DOI：
10.1093/jjco/hys236
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发表时间：
2013-03-01
影响因子：
2.4
通讯作者：
Jin, Boquan
中科院分区：
文献类型：
--
作者：
Zhou, Xingchun;Yang, Fan;Jin, Boquan
本研究的目的是检测CT10、CT45和GAGE7在肿瘤间表达的异质性，并进一步分析其预后价值。采用免疫组化方法对120例浸润性导管乳腺癌患者的肿瘤间三种癌/睾丸抗原进行了异质性分析。表达模式被分类，并与患者的临床病理变量和转归相关。CT10根据其分布特点表现为点状、局灶性和弥漫性表达。根据其亚细胞位置的不同，CT45表现为细胞质、细胞核或细胞质和细胞核联合表达模式。GAGE7表现为细胞核、细胞质和核仁表达模式。三种癌/睾丸抗原在浸润性导管乳腺癌中也有协调表达。肿瘤患者CT10表达与淋巴结转移（P < 0.001）和临床分期（P < 0.001）显著相关。肿瘤细胞质中有GAGE7和两种或两种以上癌/睾丸抗原表达的患者与晚期临床分期显著相关（P < 0.001和P < 0.030）。CT45不同表达模式与临床病理变量间无显著差异。此外，KaplanMeier分析显示，浸润性导管性乳腺癌患者预后不良与三种癌/睾丸抗原的弥漫性CT10表达和共表达有关。弥漫性CT10表达和三种癌/睾丸抗原的共表达可作为区分预后较差乳腺癌患者的生物标志物。我们的发现可能为评估这种疾病的预后和提高基于三种癌/睾丸抗原的治疗应用的有效性提供有用的数据。
The goal of this study was to detect the intertumoral heterogeneity of CT10, CT45 and GAGE7 expression and further to analyze their prognostic value.The intertumoral heterogeneity of three cancer/testis antigens was examined by immunohistochemistry using 120 samples from patients with infiltrating ductal breast carcinoma. The expression patterns were classified and correlated with the clinicopathologic variables and outcome of the patients.CT10 showed punctate, focal and diffuse expression patterns according to the characteristic of its distribution. CT45 showed cytoplasmic, nuclear or combined cytoplasmic and nuclear expression patterns according to its subcellular location. GAGE7 exhibited nuclear, cytoplasmic and nucleolar expression patterns. Three cancer/testis antigens were also observed coordinately expressed in infiltrating ductal breast carcinoma. Patients with tumors with CT10 expression was significantly correlated with nodal metastases (P 0.001) and advanced clinical stages (P 0.001). Patients with tumors with cytoplasmic GAGE7 and with the expression of two or more cancer/testis antigens were significantly correlated with advanced clinical stages (P 0.001 and P 0.030). No signicant difference was identied between the different expression patterns of CT45 and clinicopathologic variables. In addition, KaplanMeier analysis revealed that diffuse CT10 expression and coexpression of three cancer/testis antigens were related to the poor prognosis of patients with infiltrating ductal breast carcinoma.Diffuse CT10 expression and the coexpression of three cancer/testis antigens can be used as a biomarker to distinguish patients with a poorer outcome of the breast carcinoma. Our finding may provide useful data for evaluating the prognosis of this disease and improving the effectiveness of therapeutic application based on the three cancer/testis antigens.