SOLID TUMOR PREPARATION FOR CLINICAL-APPLICATION OF FLOW-CYTOMETRY
SOLID TUMOR PREPARATION FOR CLINICAL-APPLICATION OF FLOW-CYTOMETRY
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DOI：
10.1002/cyto.990080509
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发表时间：
1987-09-01
期刊：
影响因子：
--
通讯作者：
CRISSMAN, J
中科院分区：
文献类型：
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作者：
ENSLEY, JF;MACIOROWSKI, Z;CRISSMAN, J
最近在化疗和放疗的肿瘤反应方面取得的进展引起了人们对晚期头颈鳞状细胞癌（H&N SCC）的浓厚兴趣。不幸的是，使用临床参数或常规形态学不能在个体患者的基础上充分预测此类患者的反应模式和临床结果。因此，对此类肿瘤中流式细胞仪测定的细胞参数的研究很有意义，但由于肿瘤制备技术不足而受到阻碍。该杂志上一篇文章 (10) 描述了小鼠鳞状细胞癌肿瘤 LC12 的使用，用于比较测试和确定该肿瘤的最佳制备技术。本报告描述了这些技术在 144 个 H&N 人类 SCC 标本中的应用。这些样本的平均总产量是 7.4 倍。 107 个细胞/克组织。平均活酶产量（3.3×107细胞/克）高于平均活机械产量（2.0×107细胞/克），除非淋巴结是样本来源（5.4×107细胞/克）。酶解样本的平均染料排除活力高于 90%。在机械分离和去核的标本中，显着的非整倍体亚群损失是明显的。 65%的酶解标本被成功培养，平均克隆效率为2.1倍。 10-3。来自与小鼠标准肿瘤的比较测试的制备技术已成功应用于 144 个 H&N 鳞状细胞癌标本，具有高产率和优异的存活率。 LC12 初步测试期间发现的技术问题在人类肿瘤中得到了证实。
Intense interest in advanced squamous cell cancers of the head and neck (SCC of H&N) has resulted from the recent progress made in tumor responses with chemotherapy and radiotherapy. Unfortunately, the response patterns and clinical outcome of such patients are not adequately predicted on an indiviudal patient basis using clinical parameters or conventional morphology. The study of flow cytometrically determined cellular parameters in such tumors is therefore of interest, but is hindered by inadequate tumor preparative technology. The previous article (10) in this journal describes the use of a murine SCC tumor, LC12, which was employed for comparative testing and determination of optimum techniques of preparation for this tumor. This report describes the application of these techniques to 144 specimens of human SCC of H&N. The mean total yield for these specimens is 7.4 .times. 107 cells/g of tissue. The mean viable enzymatic yield (3.3 .times. 107 cells/g) was higher than the mean viable mechanical yield (2.0 .times. 107 cells/g) except when lymph nodes were the source of the specimen (5.4 .times. 107 cells/g). The mean dye exclusion viability from enzymatically dissociated specimens wre above 90%. Significant aneuploidal subpopulation losses were evident in mechnically dissociated and enuclated specimens. 65% of the enzymatically dissociated specimens were successfully cultured with a mean cloning efficiency of 2.1 .times. 10-3. Preparative techniques derived from comparative testing with a murine standard tumor have been successfully applied to 144 specimens of SCC of H&N with resultant high yields and excellent viability. Technical problems detected during the preliminary testing with LC12 were confirmed in the human tumors.