Induction of coronary arteritis with administration of CAWS (Candida albicans water-soluble fraction) depending on mouse strains
Induction of coronary arteritis with administration of CAWS (Candida albicans water-soluble fraction) depending on mouse strains
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DOI：
10.1081/iph-200042295
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Ohno, N
中科院分区：
文献类型：
--
作者：
Nagi-Miura, N;Shingo, Y;Ohno, N
腹腔注射CAWS（取自白色念珠菌培养上清液的水溶性胞外多糖）可诱导小鼠出现类似川崎病的冠状炎。我们分析了在给药第1周和第5周连续5天以4 mg/小鼠剂量注射CAWS的小鼠株C3H/HeN、C57BL/6、DBA/2和CBA/J中与冠状动脉炎发生相关的细胞因子的产生差异。C57BL/6、C3H/HeN和DBA/2小鼠动脉炎发生率均为100%，而CBA/J小鼠动脉炎发生率仅为10%。DBA/2小鼠冠状动脉炎最严重，观察期间有多只小鼠死亡。在脾细胞对CAWS的特异性反应过程中，CAWS敏感株显示IL-6和ifn - γ水平升高。相比之下，在caws耐药的CBA/J小鼠中，IL-10水平明显升高，而在caws敏感的CBA/J小鼠中则无明显升高。然而，tnf - α水平仅在DBA/2小鼠中升高。疾病发展和细胞因子产生的差异强烈表明，CAWS免疫反应的遗传背景有助于冠状动脉炎的发生。
The intraperitoneal administration of CAWS (water-soluble extracellular polysaccharide fraction obtained from the culture supernatant of Candida albicans) to mice induces coronaritis similar to Kawasaki disease. We analyzed differences in the production of cytokines involved in the occurrence of coronary arteritis among mouse strains, C3H/HeN, C57BL/6, DBA/2 and CBA/J that were injected with CAWS at 4 mg/mouse for 5 consecutive days in the first week and the fifth week of administration. The incidence of arteritis was 100% in C57BL/6, C3H/HeN and DBA/2 mice, but only 10% in CBA/J mice. The coronary arteritis observed in DBA/2 mice was the most serious, with several mice expiring during the observation period. The CAWS-sensitive strains revealed increased levels of IL-6 and IFN-gamma during the course of a specific response to CAWS by spleen cells. In contrast, IL-10 levels were observed to increase markedly in CAWS-resistant CBA/J mice, but not the CAWS-sensitive strains. However, TNF-alpha levels were more elevated only in DBA/2 mice. The difference in disease development and cytokine production strongly suggests that the genetic background of the immune response to CAWS contributes to the occurrence of coronary arteritis.