Global Identification of Genes Specific for Rice Meiosis.
Global Identification of Genes Specific for Rice Meiosis.
复制标题
水稻减数分裂特异性基因的全球鉴定。
DOI：
10.1371/journal.pone.0137399
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yu H
中科院分区：
文献类型：
--
作者：
Zhang B;Xu M;Bian S;Hou L;Tang D;Li Y;Gu M;Cheng Z;Yu H
细线期-偶线期转换是减数分裂进程中的一个重要步骤，在此过程中同源染色体之间的配对开始，双链断裂发生。OsAM 1是玉米AM 1和拟南芥SWI 1的同源基因，编码水稻减数分裂过程中由细线期向偶线期过渡所必需的一个卷曲螺旋结构域。为了更深入地了解Osam 1在水稻减数分裂中的作用并鉴定更多的减数分裂特异性基因，我们使用RNA-Seq结合生物信息学和统计分析对Osam 1突变体和野生型水稻幼穗的转录组进行了表征。结果，在野生型和Osam 1突变体植物的幼穗中分别表达了总共25，750和28，455个基因，并且鉴定了4，400个差异表达基因（DEG; log 2 Ratio ≥ 1，FDR ≤ 0.05）。在这些DEG中，检测到4个已知的水稻减数分裂特异基因，并通过将这些DEG定位到KEGG数据库中的参考生物学途径中，发现了22个新的推定的减数分裂相关基因。通过比较我们的RNA-Seq数据与拟南芥和裂殖酵母中已知的减数分裂基因，我们鉴定了另外8个高度保守的OsAM 1响应水稻减数分裂基因。
The leptotene-zygotene transition is a major step in meiotic progression during which pairing between homologous chromosomes is initiated and double strand breaks occur. OsAM1, a homologue of maize AM1 and Arabidopsis SWI1, encodes a protein with a coiled-coil domain in its central region that is required for the leptotene-zygotene transition during rice meiosis. To gain more insight into the role of OsAM1 in rice meiosis and identify additional meiosis-specific genes, we characterized the transcriptomes of young panicles of Osam1 mutant and wild-type rice plants using RNA-Seq combined with bioinformatic and statistical analyses. As a result, a total of 25,750 and 28,455 genes were expressed in young panicles of wild-type and Osam1 mutant plants, respectively, and 4,400 differentially expressed genes (DEGs; log2 Ratio ≥ 1, FDR ≤ 0.05) were identified. Of these DEGs, four known rice meiosis-specific genes were detected, and 22 new putative meiosis-related genes were found by mapping these DEGs to reference biological pathways in the KEGG database. We identified eight additional well-conserved OsAM1-responsive rice meiotic genes by comparing our RNA-Seq data with known meiotic genes in Arabidopsis and fission yeast.