Correlation of healing capacity with vascular response in the anterior cruciate and medial collateral ligaments of the rabbit
Correlation of healing capacity with vascular response in the anterior cruciate and medial collateral ligaments of the rabbit
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DOI：
10.1016/s0736-0266(03)00078-0
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发表时间：
2003-11-01
影响因子：
2.8
通讯作者：
Salo, PT
中科院分区：
文献类型：
--
作者：
Bray, RC;Leonard, CA;Salo, PT
在临床上，前交叉韧带（ACL）损伤后的功能恢复通常比内侧副韧带（MCL）损伤后差。在损伤的实验研究中，韧带愈合的早期阶段需要增加血液供应。我们假设ACL和MCL愈合特性的差异反映在它们对部分损伤的血管反应的大小上。本研究首次定量和确定兔ACL和MCL半切后血流量和血管体积变化的时间过程。成年雌性家兔分为对照组、假手术组、ACL半切组和MCL半切组。手术诱导标准化ACL或MCL损伤。术后2、6或16周，测量ACL和MCL的血流量和血管容积，结果显示，半切后，兔MCL的血流量显著增加，并出现大量的血管生成反应，伴有炎症和瘢痕形成。在随后的基质重塑过程中，血流量和血管体积恢复到对照值。相比之下，ACL显示血管体积仅增加2倍，血流量没有增加，并且在半切后萎缩。MCL通过血管生成和增加血流来增加其血液供应的上级能力对于韧带愈合的发生是必不可少的，并且可能是ACL和MCL之间愈合潜力的主要差异。(C)2003骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
In clinical terms, functional recovery after anterior cruciate ligament (ACL) injury is generally poorer than after medial collateral ligament (MCL) injury. In experimental studies of injury, the early phases of ligament healing require an augmented blood supply. We hypothesized that the differences in healing properties of the ACL and MCL would be reflected in the magnitude of their vascular responses to partial injury. This study is the first to quantify and define the time course of changes in blood flow and vascular volume following hemisection of the rabbit ACL and MCL.Adult female rabbits were assigned to control, sham operation, ACL hemisection or MCL hemisection groups. Standardized ACL or MCL injuries were surgically induced. About 2, 6 or 16 weeks later, blood flow and vascular volume of the ACL and MCL were measured.The MCL of the rabbit responded to hemisection with a large significant increase in blood flow and a substantial angiogenic response associated with inflammation and scar formation. During subsequent matrix remodelling, blood flow and vascular volume returned towards control values. In contrast, the ACL showed only a 2-fold increase in vascular volume, no increase in blood flow and atrophied after hemisection. The superior capacity of the MCL to increase its blood supply through angiogenesis and increased flow is essential for ligament healing to occur, and may be the major difference in healing potential between the ACL and MCL. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.