Specific protective activity demonstrated in eggs of broodstock salmon injected with rabbit antibodies raised against a fish pathogen
Specific protective activity demonstrated in eggs of broodstock salmon injected with rabbit antibodies raised against a fish pathogen
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DOI：
10.3354/dao031095
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发表时间：
1997-11-20
影响因子：
1.4
通讯作者：
Iwama, GK
中科院分区：
文献类型：
--
作者：
Brown, LL;Evelyn, TPT;Iwama, GK
许多传染病病原体在生命早期就影响鲑鱼，早在鱼类的免疫系统能够产生有效的保护性反应之前。这项研究是为了确定是否可以通过在产卵前将抗体注射到雌性亲鱼鲑鱼中来将保护性抗体转移到鲑鱼卵和胚胎中。对鳗弧菌（Va）的抗性用作该系统的模型，因为已知对弧菌病的抗性是抗体介导的。在产卵前将兔抗Va抗体注射到雌性银鲑Oncorhynchus kisutch中，并通过免疫测定在卵中检测到。鱼苗从注射亲鱼也挑战与Va后，他们的卵黄囊吸收，但没有保护免受Va。将来自所有亲鱼（包括盐水注射的对照）的卵的内容物注射到幼稚的银鳕鱼鱼苗中，然后用活的Va.从亲鱼注射兔抗Va抗体的鸡蛋材料赋予对Va的保护，相反，从亲鱼注射盐水的鸡蛋材料没有。结果表明，哺乳动物抗体的垂直转移可以发生在鲑科动物，然而，任何赋予的保护是不会维持很长时间后，卵黄囊被吸收。所描述的研究结果表明，被动免疫大量的卵黄囊幼虫鲑鱼对某些病原体的潜在有用的新方法。
Many infectious disease agents affect salmonids early in life, long before the immune system of the fish is able to mount an efficient, protective response. This study was undertaken to determine if protective antibodies could be transferred to salmonid eggs and embryos by injecting the antibodies into female broodstock salmon prior to spawning. Resistance to Vibrio anguillarum (Va) was used as the model for this system because it is known that resistance to vibriosis is antibody-mediated. Antibodies raised against Va in rabbits were injected into female coho salmon Oncorhynchus kisutch before spawning and were detectable in the eggs by immunoassays. Fry from the injected broodstock were also challenged with Va following absorption of their yolk sac, but were not protected against Va. Contents of eggs from all broodstock (including saline-injected controls) were injected into naive coho fry, which were then challenged with live Va. Egg material from broodstock injected with rabbit anti-Va antibodies conferred protection against Va; in contrast, egg material from broodstock injected with saline did not. The results suggest that vertical transfer of mammalian antibodies can occur in salmonids; however, any conferred protection is not maintained for long after the yolk sac is absorbed. The findings described demonstrate a potentially useful new approach for passively immunizing large numbers of yolk sac larval salmonids against certain pathogens.