ADIPOSE-TISSUE GLUCOSE TRANSPORTERS IN NIDDM - DECREASED LEVELS OF MUSCLE FAT ISOFORM

ADIPOSE-TISSUE GLUCOSE TRANSPORTERS IN NIDDM - DECREASED LEVELS OF MUSCLE FAT ISOFORM
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DOI：
10.2337/diabetes.40.4.472
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发表时间：
1991-04-01
期刊：
DIABETES
影响因子：
7.7
通讯作者：
CARO, JF
CARO, JF
中科院分区：
医学1区
文献类型：
--
作者：
SINHA, MK;RAINERIMALDONADO, C;CARO, JF

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我们从葡萄糖转运效应系统水平探讨了肥胖和非胰岛素依赖型糖尿病（NIDDM）患者脂肪组织外周胰岛素抵抗的机制。 与对照组相比，从肥胖非糖尿病和肥胖NIDDM受试者新鲜分离的脂肪细胞对葡萄糖转运刺激的胰岛素敏感性和反应性降低，与肥胖NIDDM患者相关的变化更明显。 通过用单克隆抗体1F 8对洗涤剂可溶性脂肪组织提取物进行Western印迹分析，确定三组受试者中肌肉/脂肪葡萄糖转运蛋白亚型的相对丰度。 肥胖本身对脂肪组织肌肉/脂肪葡萄糖转运蛋白亚型没有影响（对照受试者为3150 +/- 660 vs. 4495 +/- 410计数/min [cpm]/mg蛋白）。 然而，在NIDDM患者的脂肪组织中观察到肌肉/脂肪同种型的显著消耗（1560 +/-600 cpm/mg蛋白质）。 此外，NIDDM患者脂肪组织中肌肉/脂肪亚型水平的降低也反映在分离的脂肪细胞中。 我们的研究结果表明，胰岛素抵抗在分离脂肪细胞的NIDDM患者可能至少部分是由于脂肪组织肌肉/脂肪葡萄糖转运蛋白亚型的显着消耗。
We investigated the mechanism of peripheral insulin resistance in the adipose tissue of obese and non-insulin-dependent diabetes mellitus (NIDDM) patients at the level of the glucose-transport effector system. Freshly isolated adipocytes from obese nondiabetic and obese NIDDM subjects had decreased insulin sensitivity and responsiveness for glucose-transport stimulation compared with control subjects, with more pronounced changes associated with obese NIDDM patients. The relative abundance of muscle/fat glucose-transporter isoform in the three groups of subjects was determined by Western-blot analysis of detergent-soluble adipose tissue extracts with monoclonal antibody 1F8. Obesity per se had no effect on adipose tissue muscle/fat glucose-transporter isoform (3150 +/- 660 vs. 4495 +/- 410 counts/min [cpm]/mg protein in control subjects). However, significant depletion of muscle/fat isoform was observed in adipose tissue of NIDDM patients (1560 +/- 600 cpm/mg protein). Furthermore, decreased levels of muscle/fat isoform in adipose tissue of NIDDM patients were also reflected in isolated adipocytes. Our results demonstrate that insulin resistance in isolated adipocytes of NIDDM patients could at least partly be due to a significant depletion of adipose tissue muscle/fat glucose-transporter isoform.