Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells
Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells
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DOI：
10.1242/jcs.03204
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发表时间：
2006-11-01
影响因子：
4
通讯作者：
Giet, Regis
中科院分区：
文献类型：
--
作者：
Delcros, Jean-Guy;Prigent, Claude;Giet, Regis
动力蛋白复合体与动力蛋白复合体在各种系统中协同作用，完成有丝分裂。在这里，我们分析了果蝇S2细胞的有丝分裂表型的动力蛋白亚基p150（Glued）敲低。我们发现，p150（Glued）-耗尽的细胞在中期延迟，中心体连接不良有丝分裂纺锤体极。此外，后期发生了异步染色体分离。虽然细胞周期蛋白B在这些后期细胞中降解，但Aurora B、MEI-S322和BubR 1没有从非分离染色体中释放。我们还发现，中央纺锤体的密度和组织受到损害，与极光B和波罗激酶缺席的微管数量减少。Pavarotti-KLP是形成稳定微管束所需的centralspindlin复合物的一种成分，在后期开始后，没有立即靶向微管的正端，如对照组中所发生的那样。相反，它在细胞分裂后期早期在细胞皮层短暂积累，其靶向中央纺锤体被延迟。这些数据表明，dynactin复合物有助于胞质分裂，促进稳定的目标centralspindlin复合物微管加结束在后期开始。的动力蛋白-dynactin复杂的同步染色体分离和胞质分裂的贡献进行了讨论。
The dynactin complex cooperates with the dynein complex in various systems for mitotic completion. Here we analysed the mitotic phenotype of Drosophila S2 cells following the knockdown of the dynactin subunit p150(Glued). We found that p150(Glued)-depleted cells were delayed in metaphase and that the centrosomes were poorly connected to mitotic spindle poles. In addition, anaphase occurred with asynchronous chromosome segregation. Although cyclin B was degraded in these anaphase cells, Aurora B, MEI-S322 and BubR1 were not released from the non-segregating chromosomes. We also found that the density and organisation of the central spindle were compromised, with Aurora B and polo kinases absent from the diminished number of microtubules. Pavarotti-KLP, a component of the centralspindlin complex required for the formation of stable microtubule bundles, was not immediately targeted to the plus ends of the microtubules following anaphase onset as happened in controls. Instead, it accumulated transiently at the cell cortex during early anaphase and its targeting to the central spindle was delayed. These data suggest that the dynactin complex contributes to cytokinesis by promoting stable targeting of the centralspindlin complex to microtubule plus ends at anaphase onset. The contribution of the dynein-dynactin complex to synchronous chromosome segregation and cytokinesis is discussed.