Interaction of [Ru(bpy)2(dppz)]2+ with human telomeric DNA: Preferential binding to G-quadruplexes over i-motif
Interaction of [Ru(bpy)2(dppz)]2+ with human telomeric DNA: Preferential binding to G-quadruplexes over i-motif
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[Ru(bpy)2(dppz)]2 与人端粒 DNA 的相互作用：优先结合 G-四链体而不是 i-基序
DOI：
10.1016/j.biochi.2010.01.003
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发表时间：
2010-04-01
期刊：
影响因子：
3.9
通讯作者：
Ji, Liangnian
中科院分区：
文献类型：
--
作者：
Shi, Shuo;Geng, Xiaoting;Ji, Liangnian
受端粒DNA结构和功能的重要性的启发，我们将注意力集中在[Ru（bpy）（2）（dPPz）（2+）]与富含鸟嘌呤的单链寡聚体5 '-AGGGTTAGGGTTAGGGTTAGGG-3'（22 AG）和互补的富含胞嘧啶的单链（22 CT）的相互作用上.在Na+缓冲液中，22 AG可能呈反平行篮状四链体结构，而在K+缓冲液中，22 AG则呈平行/反平行混合结构。22 CT可以在酸性pH下自缔合成i基序。本文研究了[Ru（bpy）（2）（dPPz）（2+）]与各种DNA的相互作用。有趣的是，[Ru（bpY）（2）（dPPz）（2+）]能在不加任何阳离子的情况下促进人端粒重复序列22 AG折叠成分子内反平行的G-四链体。此外，[Ru（bpy）（2）（dppz）（2+）]对Na+或K+诱导的G-四链体有较强的结合选择性，而对i-基序的结合选择性较弱。研究结果还表明，[Ru（bpY）（2）（dPPz）（2+）]可以作为两种G-四链体的一个重要的分子“光开关”，揭示了标题配合物在G-四链体DNA发光信号转导中的潜在应用。(C)2010年Elsevier Masson SAS。All rights reserved.
Inspired by the enormous importance attributed to the structure and function of human telomeric DNA, we focus our attention on the interaction of [Ru(bpy)(2)(dPPz)(2+) with the guanine-rich single-strand oligomer 5'-AGGGTTAGGGTTAGGGTTAGGG-3' (22AG) and the complementary cytosine-rich strand (22CT). In Na+ buffer, 22AG may adopt an antiparallel basket quadruplex, whereas, it favours a mixed parallel/antiparallel structure in K+ buffer. 22CT may self-associate at acidic pH into an i-motif. In this paper, the interaction between [Ru(bpy)(2)(dPPz)(2+) and each unusual DNA was evaluated. It was interesting that [Ru(bpY)(2)(dPPz)(2+) could promote the human telomeric repeat 22AG to fold into intramolecular antiparallel G-quadruplex without any other cations. What's more, [Ru(bpy)(2)(dppz)(2+)] was found to have a strong preference for binding to G-quadruplexes that were induced through either Na+ or K+, while weak binding to i-motif was observed. The results also indicated that [Ru(bpY)(2)(dPPz)(2+) could serve as a prominent molecular "light switch" for both G-quadruplexes, revealing a potential application of the title complex in luminescent signaling of G-quadruplex DNA. (C) 2010 Elsevier Masson SAS. All rights reserved.