Protein loss during nuclear isolation.
Protein loss during nuclear isolation.
复制标题
核分离过程中的蛋白质损失。
DOI：
10.1083/jcb.97.4.1240
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发表时间：
1983-10
影响因子：
7.8
通讯作者：
Horowitz, S B
中科院分区：
文献类型：
--
作者：
Paine, P L;Austerberry, C F;Desjarlais, L J;Horowitz, S B
冷冻显微解剖使得测量两栖动物卵母细胞核的整个体内蛋白质含量成为可能，并提供了一个迄今为止缺失的基线，用于估计蛋白质损失在核分离过程中通过其他方法。当卵母细胞核被分离到水性介质中时，它们在250 s的半衰期内失去95%的蛋白质。这一结果意味着，从正常大小的细胞的含水分离的细胞核中，蛋白质的损失甚至更快。
Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells.