Conserved features of non-primate bilaminar disc embryos and the germline.
Conserved features of non-primate bilaminar disc embryos and the germline.
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DOI：
10.1016/j.stemcr.2021.03.011
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发表时间：
2021-05-11
影响因子：
5.9
通讯作者：
Surani MA
中科院分区：
文献类型：
--
作者：
Alberio R;Kobayashi T;Surani MA
大多数哺乳动物（包括人类）的胚胎着床后发育开始于双椎板。尽管对早期人类胚胎的研究受到限制，并受到更严格的伦理审查，但对作为双层椎间盘发育的非灵长类胚胎的研究为原肠胚形成、种系和适用于人类发育的进化分歧的基础提供了特殊的机会。在这里，我们讨论了在猪胚胎中作为与早期人类发育相关的双层椎间盘胚胎发育范例的研究的优势。此外，这种猪还具有创造用于异种移植的人源化器官的潜力。精确的基因工程方法、成像和单细胞分析具有成本效益和效率，能够研究人类发育的一些突出问题，并开发出具有干细胞的早期人类发育的真实模型。Alberio等人讨论了适用于人类的非灵长类动物的早期发育和生殖系起源的保守特征，其优点是更容易获得和较少的伦理问题。单细胞分辨率分析和基因操作将为开发早期人类发育的真实体外模型提供对原肠哺乳动物胚胎进化的深入了解。
Post-implantation embryo development commences with a bilaminar disc in most mammals, including humans. Whereas access to early human embryos is limited and subject to greater ethical scrutiny, studies on non-primate embryos developing as bilaminar discs offer exceptional opportunities for advances in gastrulation, the germline, and the basis for evolutionary divergence applicable to human development. Here, we discuss the advantages of investigations in the pig embryo as an exemplar of development of a bilaminar disc embryo with relevance to early human development. Besides, the pig has the potential for the creation of humanized organs for xenotransplantation. Precise genetic engineering approaches, imaging, and single-cell analysis are cost effective and efficient, enabling research into some outstanding questions on human development and for developing authentic models of early human development with stem cells. Alberio et al. discuss conserved features of early development and the genesis of the germline in non-primates applicable to humans, with advantages of greater accessibility and lesser ethical concerns. The analysis at the resolution of single cells and genetic manipulations will provide insight into the evolution of gastrulating mammalian embryos for developing authentic in vitro models of early human development.
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