Disulfide-Crosslinked Biomimetic Micelles: Formation, Thiol Reactivity and Cytotoxicity Behavior
Disulfide-Crosslinked Biomimetic Micelles: Formation, Thiol Reactivity and Cytotoxicity Behavior
复制标题
二硫键交联仿生胶束：形成、硫醇反应性和细胞毒性行为
DOI：
10.1002/macp.201000458
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发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Ji, Jian
中科院分区：
文献类型：
--
作者：
Lv, Li-Ping;Xu, Jian-Ping;Ji, Jian
合成了一系列以MPC为亲水链段、SMA为疏水链段、GMA为反应链段的两亲性无规共聚物。通过调节共聚物中亲水/疏水链段的比例，得到了不同尺寸的聚合物胶束。用核磁共振氢谱、荧光探针法和差示扫描量热法研究了胶束的形成和性质。用双官能团试剂对反应性聚合物胶束进行了交联。该交联胶束具有更好的稳定性和硫醇反应活性。细胞活性测试表明，基于生物激发的磷胆碱胶束具有良好的生物相容性，在纳米载体系统领域具有巨大的应用潜力。
A series of amphiphilic random copolymers with MPC as the hydrophilic segment, SMA as the hydrophobic segment and GMA as the reactive segment were synthesized. Polymeric micelles with different sizes were obtained through tuning the hydrophilic/hydrophobic segment ratios in the copolymers. The formation and properties of these micelles were investigated using H-1 NMR spectroscopy, fluorescence probe techniques and DLS. The reactive polymeric micelles were crosslinked with a di-functional reagent. The crosslinked micelles showed improved stability and thiol reactivity. Cell viability tests indicated that the bioinspired phosphorylcholine-based micelles showed excellent biocompatibility, thus promising great potential for applications in the field of nanocarrier systems.