Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR
Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR
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DOI：
10.1002/app.20647
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发表时间：
2004-08-15
影响因子：
3
通讯作者：
Miranda, SP
中科院分区：
文献类型：
--
作者：
Cárdenas, G;Cabrera, G;Miranda, SP
报告了从鱿鱼、虾、对虾、龙虾和帝王蟹中获得的甲壳素的完整特征。进行了元素分析，包括 Ca、Mg、Zn、Cd、Hg、Cr、Mn、Cu 和 Pb 等金属，这是非常相关的，因为每个物种的骨架组成略有不同。通过 TEM 研究形态，并通过能量色散 X 射线分析确定其组成。应用 C-13 交叉极化/魔角旋转 NMR 来确定所有碳的化学位移以及它们之间的差异。采用化学方法，交替使用盐酸和氢氧化钠来分离甲壳素。来自虾、对虾、龙虾和帝王蟹的 α-几丁质在 73.7 和 75.6 ppm 处显示出两个信号。与此同时，来自鱿鱼的 β-几丁质在 75.2 ppm 处显示出一个信号。 FTIR 研究用于分析虾中的 a-几丁质和鱿鱼中的 β-几丁质。 α-甲壳素在1660和1627 cm(-1)处显示酰胺I振动模式，而β-甲壳素在1656 cm(-1)处显示一个谱带。 X 射线衍射表明，α-几丁质是斜方晶系（a = 4.74 埃，b = 18.86 埃，c 10.32 埃），β-几丁质具有单斜二水合物形式（a = 4.80 埃，b = 10.40 埃，c = 11.10 埃，β = 97）。 (C) 2004 年 Wiley 期刊公司。
The full characterization of chitin obtained from squid, shrimp, prawn, lobsters, and king crab is reported. Elemental analysis, including metals such as Ca, Mg, Zn, Cd, Hg, Cr, Mn, Cu, and Pb, was performed, which is quite relevant because the skeleton composition is slightly different for each species. The morphology was studied by means of TEM and their compositions were determined by energy-dispersive X-ray analysis. C-13 cross polarization/magic angle spinning NMR was applied to determine the chemical shift of all the carbons and the difference between them. Chitin was isolated by using chemical methods, alternating hydrochloric acid and sodium hydroxide. The alpha-chitin from shrimp, prawn, lobsters, and king crabs showed two signals at 73.7 and 75.6 ppm. Meanwhile, the beta-chitin from squid exhibited one signal at 75.2 ppm. FTIR studies were used to analyze a-chitin from shrimp and beta-chitin from squid. The a-chitin exhibited amide I vibration modes at 1660 and 1627 cm(-1), whereas the beta-chitin showed one band at 1656 cm(-1). X-ray diffraction showed that alpha-chitin is orthorhombic (a = 4.74 Angstrom, b = 18.86 Angstrom, and c 10.32 Angstrom) and beta-chitin had a monoclinic dihydrated form (a = 4.80 Angstrom, b = 10.40 Angstrom, c = 11.10 Angstrom, and beta = 97). (C) 2004 Wiley Periodicals, Inc.