Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions
Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions
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DOI：
10.1093/pcp/pcf156
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发表时间：
2002-10-01
影响因子：
4.9
通讯作者：
Yano, M
中科院分区：
文献类型：
--
作者：
Kojima, S;Takahashi, Y;Yano, M
抽穗期3a（Hd 3a）是水稻品种日本晴和Kasalath杂交中与抽穗期相关的数量性状位点。先前的研究表明，Hd 3a的Kasalath等位基因在短日照（SD）条件下促进抽穗。高分辨率连锁图谱将Hd 3a定位在一个类似于20-kb的基因组区域。在该区域，我们发现了一个候选基因，该基因与拟南芥中促进开花的FLOWARDINLOCUS T（177）基因具有高度相似性。该基因的导入在水稻中引起了早抽穗表型。在SD条件下，Hd 3a的转录水平增加。水稻抽穗期1（Hd 1）基因是CONSTANS（CO）基因的同源基因，在SD条件下具有促进抽穗的作用。通过表达分析，我们发现，在SD条件下，Hd 3a mRNA的量被Hd 1上调，这表明Hd 3a在Hd 1的控制下促进抽穗。这些结果表明Hd 3a编码与拟南芥FT密切相关的蛋白，并且Hd 3a和FT的功能和与Hd 1和CO的调控关系分别在水稻（SD植物）和拟南芥（长日植物）之间是保守的。
Heading date 3a (Hd3a) has been detected as a heading-date-related quantitative trait locus in a cross between rice cultivars Nipponbare and Kasalath. A previous study revealed that the Kasalath allele of Hd3a promotes heading under short-day (SD) conditions. High-resolution linkage mapping located the Hd3a locus in a similar to20-kb genomic region. In this region, we found a candidate gene that shows high similarity to the FLOWERING LOCUS T (177) gene, which promotes flowering in Arabidopsis. Introduction of the gene caused an early-heading phenotype in rice. The transcript levels of Hd3a were increased under SD conditions. The rice Heading date 1 (Hd1) gene, a homolog of CONSTANS (CO), has been shown to promote heading under SD conditions. By expression analysis, we showed that the amount of Hd3a mRNA is up-regulated by Hd1 under SD conditions, suggesting that Hd3a promotes heading under the control of Hd1. These results indicate that Hd3a encodes a protein closely related to Arabidopsis FT and that the function and regulatory relationship with Hd1 and CO, respectively, of Hd3a and FT are conserved between rice (an SD plant) and Arabidopsis (a long-day plant).