Coculture with a Human Granulosa Cell Line Enhanced the Development of Murine Preimplantation Embryos via SCF/c-Kit System
Coculture with a Human Granulosa Cell Line Enhanced the Development of Murine Preimplantation Embryos via SCF/c-Kit System
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通过 SCF/c-Kit 系统与人颗粒细胞系共培养增强小鼠植入前胚胎的发育
DOI：
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发表时间：
2004
影响因子：
3.1
通讯作者：
N. Terakawa
中科院分区：
文献类型：
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作者：
F. Taniguchi;T. Harada;M. Nara;I. Deura;M. Mitsunari;N. Terakawa
摘要目的：探讨小鼠胚胎与人卵巢颗粒瘤源性细胞系（KGN细胞）共培养的效果。
AbstractPurpose: To evaluate the effect on the coculture of murine embryos with a human ovarian granulosa tumor derived cell line (KGN cells).
Methods: We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem cell factor (SCF). The reverse transcriptase–polymerase chain reaction (RT-PCR) method was used to analyze the gene expression of SCF in KGN cells cocultured with murine embryos.
Results: The coculture system with KGN cells significantly increased the rate of embryo development to late blastocyst and to hatching stage. We also found that coculture with murine embryos enhanced the gene expression of SCF in KGN cells. Adding human recombinant SCF to the medium significantly enhanced embryo development to late blastocyst and hatching stage.
Conclusions: KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine system.
小鼠胚胎与从人卵泡、输卵管和子宫内膜分离的细胞共培养。
影响因子：
6.7
作者：
Freeman,MR;Bastias,MC;Hill,GA;Osteen,KG
通讯作者：
Osteen,KG
影响因子：
2.7
作者：
ARCECI, RJ;PAMPFER, S;POLLARD, JW
通讯作者：
POLLARD, JW