Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro.
Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro.
复制标题
DOI：
10.1007/7651_2016_319
复制
发表时间：
2016
影响因子：
--
通讯作者：
Y. Ouji;M. Yoshikawa
中科院分区：
文献类型：
--
作者：
Y. Ouji;M. Yoshikawa
毛乳头细胞（DPC）与毛囊（HF）的发育和头发周期的调节有关。然而，已知原代DPC在用于成纤维细胞的标准培养基中培养后丧失其诱导HF的能力。我们研究了一种新的DPC培养条件，包括添加Wnt-10 b，其促进增殖并保持其HF诱导能力超过10代。这些结果表明，Wnt-10 b在体外DPC的增殖和维持中起着关键作用。
Dermal papilla cells (DPCs) are associated with development of hair follicles (HFs) and regulation of the hair cycle. However, primary DPCs are known to lose their ability to induce HFs after culture in standard media for fibroblasts. We examined a new culture condition for DPCs including addition of Wnt-10b, which promoted proliferation and maintained their HF induction ability for more than ten passages. These results suggest that Wnt-10b plays a pivotal role in proliferation and maintenance of DPCs in vitro.