Chemical profiling and biological screening of Thymus lotocephalus extracts obtained by supercritical fluid extraction and hydrodistillation
Chemical profiling and biological screening of Thymus lotocephalus extracts obtained by supercritical fluid extraction and hydrodistillation
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DOI：
10.1016/j.indcrop.2011.09.014
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发表时间：
2012-03-01
影响因子：
5.9
通讯作者：
Romano, Anabela
中科院分区：
文献类型：
--
作者：
Costa, Patricia;Goncalves, Sandra;Romano, Anabela
分别采用水蒸气蒸馏法和超临界流体萃取法从红头莲地上部分中提取挥发油和提取物。SFE在40 ° C和12或18 MPa的工作压力下进行。使用GC-FID和GC-IT-MS的化学配置文件进行了测定。含氧单萜类化合物是在精油和SFE提取物中收集的第二个分离器的主要成分，而在第一个分离器中获得的提取物主要是含氧倍半萜。大量的化合物进行了鉴定，通过水蒸气蒸馏，相比之下，最高的提取率得到使用超临界流体萃取。芳樟醇（10.43 +/- 1.63%）是精油中的主要成分，而樟脑（7.91 +/- 0.84%）和顺式芳樟醇氧化物（7.25 +/- 1.45%）是分别在12和18 MPa下获得的提取物-第二分离器中的主要化合物。在浸提液-第1个分离器中鉴别出的主要成分为环氧乙烷（在12和18 MPa下分别获得4.34 +/- 0.51和4.41 +/- 1.25%）。采用ORAC法和DPPH法测定其抗氧化活性，采用Ellman法测定其体外抗胆碱酯酶活性。采用超临界流体萃取法（SFE）对毛蕊花挥发油和超临界流体萃取物（第一级分离物）进行了研究。莲莲具有抗氧化活性，对胆碱酯酶有较强的抑制作用。我们还表明，乙酰胆碱酯酶和丁酰胆碱酯酶的抑制活性的精油可以归因于1，8-桉叶素和氧化香芹烯，分别。(C)2011 Elsevier B. V.保留所有权利。
Essential oil and extracts from the aerial parts of Thymus lotocephalus were obtained by hydrodistillation (HD) and supercritical fluid extraction (SFE) in two different collectors, respectively. SFE was conducted at 40 degrees C and a working pressure of 12 or 18 MPa. The chemical profiles were determined using GC-FID and GC-IT-MS. Oxygen-containing monoterpenes were the primary constituents in the essential oil and SFE extracts collected in the second separator, while the extracts obtained in the first separator were predominantly oxygen-containing sesquiterpenes. A large number of compounds were identified by hydrodistillation and, in contrast, the highest extraction yields were obtained using SFE. Linalool (10.43 +/- 1.63%) was the main component in essential oil, whereas camphor (7.91 +/- 0.84%) and cis-linalool oxide (7.25 +/- 1.45%) were the major compounds in the extracts-2nd separator obtained at pressures of 12 and 18 MPa, respectively. Caryophyllene oxide was the primary constituent identified in the extracts-1st separator (4.34 +/- 0.51 and 4.41 +/- 1.25% obtained at 12 and 18 MPa, respectively). The antioxidant activity was assessed by ORAC and DPPH assays, and the anti-cholinesterase activity was evaluated in vitro using Ellman's method. The essential oil and SFE extracts (first separator) of T. lotocephalus possessed antioxidant activity and strongly inhibited cholinesterases. We also demonstrated that the acetylcholinesterase and butyrylcholinesterase inhibitory activities of the essential oil could be attributed to 1,8-cineole and caryophyllene oxide, respectively. (C) 2011 Elsevier B.V. All rights reserved.