A mouse model of allogeneic corneal endothelial cell transplantation
A mouse model of allogeneic corneal endothelial cell transplantation
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DOI：
10.1097/qai.0b013e31815e722b
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发表时间：
2008-07-01
期刊：
影响因子：
2.8
通讯作者：
Mizuki, Nobuhisa
中科院分区：
文献类型：
--
作者：
Hayashi, Takahiko;Yamagami, Satoru;Mizuki, Nobuhisa
目的：角膜内皮细胞移植有望在临床上得到应用。然而，allo-CEC移植后的免疫学变化目前知之甚少。方法：将苯扎氯铵注射入受体BALB/c小鼠眼前房，建立大泡性角膜病变模型。采用4种角膜进行全层角膜移植：BALB/c角膜（同种移植组）、去内皮的BALB/c角膜（无内皮组）、C3 H/He小鼠角膜（同种异体移植组）和将永生化C3 H/He小鼠CEC接种到去内皮的BALB/c角膜上重建的角膜（CEC同种异体移植组）。结果：同种异体角膜移植组（n = 12）植片均透明，无内皮移植组（n = 13）植片均透明。CEC同种异体移植物组（n = 12）中75%的角膜移植物在4周时透明。CEC移植组的组织学排斥反应发生率为0%，明显低于同种异体移植组（67%; n = 18; P < 0.01）。该模型可用于研究CEC移植后的免疫学过程。
Purpose: Corneal endothelial cell (CEC) transplantation should become clinically applicable in the near future. However, the immunologic changes after allo-CEC transplantation are poorly understood at present. We tried to establish a mouse model of allogeneic CEC transplantation for immunologic studies.Methods: Benzalkonium chloride was injected into the anterior chamber of the eyes of recipient BALB/c mice to create bullous keratopathy. Full-thickness corneal transplantation was performed by using 4 types of corneas: BALB/c corneas (isograft group), BALB/c corneas denuded of CEC (no endothelium group), C3H/He mouse corneas (allograft group), and corneas reconstituted by seeding immortalized C3H/He mouse CECs onto BALB/c corneas denuded of endothelium, (CEC allograft group). Eyes were observed with an operating microscope for 4 weeks after transplantation and were subjected to histologic examination and fluorescein microscopy.Results: All corneal grafts were transparent in the isograft group (n = 12), whereas none of the grafts were clear by 4 weeks after transplantation in the no endothelium group (n = 13). Corneal grafts were transparent at 4 weeks in 75% of the CEC allograft group (n = 12). The histologic rejection rate was 0% in the CEC allograft group, which was significantly lower than in the allograft group (67%; n = 18; P < 0.01).Conclusions: We established a mouse allo-CEC transplantation model by using cultured cells. This model should be useful for studying the immunologic processes after CEC transplantation.