NADPH as a potential intrinsic probe for tumour margin estimation
NADPH as a potential intrinsic probe for tumour margin estimation
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DOI：
10.1117/12.2287414
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发表时间：
2018-03
期刊：
影响因子：
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通讯作者：
Hazel L Stewart;T. Hupp;D. Birch
中科院分区：
文献类型：
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作者：
Hazel L Stewart;T. Hupp;D. Birch
为了评估还原型辅酶NADH及其磷酸化衍生物（NADPH）作为癌症手术内在探针的潜力，人们对它们的荧光特性进行了研究。癌细胞中NADPH的生成会增加，以清除活性氧并满足更高的生物合成需求，并且它具有吸引人的荧光特性，例如在光谱的可见光部分有发射，以及与酶结合时具有相对较长的荧光寿命（约1 - 6.5纳秒），这有助于区分其他内源性物质。本文报道了不同环境对NAD(P)H荧光的影响，包括去除氧气后荧光寿命增加、在复杂介质（硅胶模型）中能够保持其荧光特性以及在细胞环境中其荧光寿命也可区分。此外，本文还报道了一种基于微型液体光导滤光片的时间相关单光子计数荧光寿命系统的开发，这是朝着癌症手术中时间分辨视觉成像迈出的一步。该系统已被证明能够准确测量NAD(P)H在简单溶剂和细胞环境中的荧光寿命。
The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.