Detection of neuron specific enolase concentrations in cerebrospinal fluid from patients with neurological disorders by means of a sensitive enzyme immunoassay.
Detection of neuron specific enolase concentrations in cerebrospinal fluid from patients with neurological disorders by means of a sensitive enzyme immunoassay.
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DOI：
10.1016/0009-8981(90)90332-m
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发表时间：
1990-02
期刊：
影响因子：
--
通讯作者：
K. Vermuyten;A. Lowenthal;D. Karcher
中科院分区：
文献类型：
--
作者：
K. Vermuyten;A. Lowenthal;D. Karcher
建立了一种检测脑脊液中神经元特异性烯醇化酶（NSE）的酶联免疫吸附试验（ELISA）。ELISA的灵敏度小于1 μg/ml。这种灵敏度可与放射免疫测定相媲美，放射免疫测定的缺点是使用放射性标记的产品。该方法用于测量1178例神经系统疾病患者的脑脊液神经元特异性烯醇化酶（CSF-NSE）水平，以确定其潜在的有用性和临床应用。本组患者CSF-NSE水平与性别无关，与年龄无关。CSF-NSE在Creutzfeldt-Jacob病、脑膜出血、血栓形成、格林-巴罗综合征和精神分裂症中显著升高。
An enzyme linked immunosorbent assay (ELISA) for the detection of neuron specific enolase (NSE) in cerebrospinal fluid (CSF) was developed. The sensitivity of the ELISA was less than 1 μg/ml. This sensitivity is comparable with radioimmunoassays which have the disadvantage that radiolabelled products are used. The developed assay was used to measure cerebrospinal fluid neuron specific enolase (CSF-NSE) levels in 1178 patients with neurological disorders to establish its potential usefulness and clinical application. CSF-NSE levels in this group of patients were independent of sex and no correlation with age was found. CSF-NSE was significantly increased in Creutzfeldt-Jacob disease, meningeal hemorrhage, thrombosis, Guillain-Barré syndrome and in schizophrenia.