Isolation and functional characterization of fatty acid Δ5-elongase gene from the liverwort Marchantia polymorpha L.
Isolation and functional characterization of fatty acid Δ5-elongase gene from the liverwort Marchantia polymorpha L.
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DOI：
10.1016/j.febslet.2005.11.065
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发表时间：
2006-01-09
期刊：
影响因子：
3.5
通讯作者：
Kohchi, T
中科院分区：
文献类型：
--
作者：
Kajikawa, M;Yamato, KT;Kohchi, T
苔藓植物地钱 (Marcantia polymorpha L.) 产生 C22 极长链多不饱和脂肪酸 (VLCPUFA)。迄今为止，尚未在陆地植物中鉴定出介导 C20 VLCPUFA 伸长的酶。在这里，我们报告了 MpELO2 基因的分离和表征，该基因编码地钱中的 ELO 样脂肪酸延长酶。酵母中的异源表达表明，MpELO2 编码 Delta 5-延伸酶，可介导花生四烯酸 (20:4) 和二十碳五烯酸 (20:5) 的延伸。系统发育和基因结构分析表明，MpELO2 基因与苔藓植物用于 C18 脂肪酸延伸的 Delta 6 延伸酶基因密切相关，并通过局部基因复制与它们分离。 (c) 2005 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Bryophyte Marchantia polymorpha L. produces C22 very-long-chain polyunsaturated fatty acid (VLCPUFA). Thus far, no enzyme that mediates elongation of C20 VLCPUFAs has been identified in land plants. Here, we report the isolation and characterization of the gene MpELO2, which encodes an ELO-like fatty acid elongase in M. polymorpha. Heterologous expression in yeast demonstrated that MpELO2 encodes Delta 5-elongase, which mediates elongation of arachidonic (20:4) and eicosapentaenoic acids (20:5). Phylogenetic and gene structural analysis indicated that the MpELO2 gene is closely related to bryophyte Delta 6-elongase genes for C18 fatty acid elongation and diverged from them by local gene duplication. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.