Assessment of chromosome 8 copy number in cervical cancer by fluorescent in situ hybridization
Assessment of chromosome 8 copy number in cervical cancer by fluorescent in situ hybridization
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DOI：
10.1006/exmp.1999.2256
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发表时间：
1999-06-01
影响因子：
3.6
通讯作者：
Lathrop, J
中科院分区：
文献类型：
--
作者：
Mark, HFL;Feldman, D;Lathrop, J
宫颈癌是一种恶性肿瘤，通常发生在鳞状上皮和腺上皮之间的转移区。绝大多数属于两种组织学类型，鳞状细胞癌和腺癌。为了鉴定以8号染色体三体为特征的子集宫颈癌，我们对福尔马林固定的石蜡包埋的宫颈癌材料进行了研究。从罗德岛医院的档案中共确认了24例宫颈癌病例。用8号染色体着丝粒计数探针进行荧光原位杂交(FISH)，检测标本中8号染色体的拷贝数。杂交信号在肿瘤细胞间以盲法计分。细胞数大于或等于15%且有三个信号的肿瘤被评为三体。在研究的24个案例中，有23个是信息性的。在23例信息性病例中，12例(52.2%)为三体。11例(47.8%)为二体。对照17号染色体探针的三体频率为13.0%(3/23)。文中还对肿瘤的临床病理特征进行了综述。8三体在浸润性鳞癌中的发生率为44.4%(8/18)，在浸润性腺癌中为80%(4/5)。唯一一例HER-2/neu癌基因扩增均为8三体的肿瘤为浸润性腺癌。虽然这项初步研究的样本量不大，但到目前为止获得的数据清楚地表明，FISH是检测染色体三体的合适技术，并且存在以8号染色体三体为特征的子宫颈癌。这一生物标志物的进一步探索是有必要的。(C)1999年学术出版社。
Cervical carcinoma is a malignancy which typically occurs at the transformation zone between squamous and glandular epithelium. The vast majority falls into two histologic types, squamous cell and adenocarcinoma. In an effort to identify a subset of cervical cancer characterized by chromosome 8 trisomy, a biomarker extensively explored by this laboratory, we conducted a study of formalin-fixed, paraffin-embedded materials of cervical cancer. A total of 24 cases of cervical cancer were identified from the archives of the Rhode Island Hospital. Fluorescent in situ hybridization (FISH) using a chromosome 8 centromere enumeration probe was conducted to assess the chromosome 8 copy number in these specimens. Hybridization signals were scored among tumor cells in a blinded fashion. Tumors with greater than or equal to 15% of cells with three signals were scored as trisomic. Of 24 cases studied, 23 were informative. Of the 23 informative cases, 12 (52.2%) were found to be trisomic. Eleven cases (47.8%) were disomic. The frequency of trisomy in a control chromosome 17 probe was 13.0% (3/23). Selected clinicopathologic characteristics of the tumors were also reviewed. The frequency of trisomy 8 among cases of invasive squamous cell carcinoma was 44.4% (8 of 18 tumors) and that of invasive adenocarcinoma was 80% (4 of 5 tumors). The sole tumor which was both trisomic 8 and amplified for the HER-2/neu oncogene was found to be an invasive adenocarcinoma. While the sample size in this pilot study is not large, the data obtained thus far clearly demonstrate that FISH is an appropriate technique for detecting chromosomal trisomies and that a subset of cervical cancer exists that is characterized by chromosome 8 trisomy. Further exploration of this biomarker is warranted. (C) 1999 Academic Press.