Ginsenoside Rb1 improves postoperative fatigue syndrome by reducing skeletal muscle oxidative stress through activation of the PI3K/Akt/Nrf2 pathway in aged rats
Ginsenoside Rb1 improves postoperative fatigue syndrome by reducing skeletal muscle oxidative stress through activation of the PI3K/Akt/Nrf2 pathway in aged rats
复制标题
人参皂苷 Rb1 通过激活老年大鼠的 PI3K/Akt/Nrf2 通路来减少骨骼肌氧化应激，从而改善术后疲劳综合征。
DOI：
10.1016/j.ejphar.2014.06.040
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发表时间：
2014-10-05
影响因子：
5
通讯作者：
Yu, Zhen
中科院分区：
文献类型：
--
作者：
Zhuang, Cheng-Le;Mao, Xiang-Yu;Yu, Zhen
人参皂苷Rb 1具有抗疲劳活性，但其作用机制尚不清楚。本研究旨在探讨人参皂苷Rb 1对老年大鼠小肠切除术后疲劳综合征（MSfR）的抗疲劳作用及其分子机制。老年MSfR大鼠从术前3天起至处死前每天1次给予15 mg/kg的Rb_1，或相应的生理盐水作为对照。无N1SfR但经历相同手术程序的大鼠给予生理盐水作为空白对照。通过旷场实验评价其抗疲劳作用;测定骨骼肌超氧化物歧化酶、活性氧和丙二醛的含量。实时定量PCR检测骨骼肌中Akt 2和NEU的mRNA水平。用Western blot和荧光法检测Akt和Nr 12的活化。结果表明，在MSIR老年大鼠中，Rb 1显著增加了运动距离和直立频率，减少了休息时间。此外，Rb 1还能显著降低MSIR老年大鼠骨骼肌中活性氧和丙二醛的释放，提高超氧化物歧化酶的活性。人参皂苷Rb 1还可增加Akt 2和NrI 2 mRNA的表达，上调Akt磷酸化和Nrf 2核转位。结论：人参皂苷Rb 1对老年大鼠术后疲劳综合征具有抗疲劳作用，其机制可能与激活PI 3 K/Akt信号通路，促进Ni 42核转位，诱导抗氧化酶活性有关。(C)2014爱思唯尔有限公司版权所有。
Ginsenoside Rb1 is reported to possess anti-fatigue activity, but the mechanisms remain unknown. The aim of this study was to investigate the molecular mechanisms responsible for the anti-fatigue effect of ginsenoside Rb1 on postoperative fatigue syndrome induced by major small intestinal resection (MSfR) in aged rat. Aged rats with MSfR were administrated with ginsenoside Rb1 (15 mg/kg) once a day from 3 days before surgery to the day of sacrifice, or with saline as corresponding controls. Rats without N1SfR but going through the same surgery procedure were administrated with saline as blank controls. Antifatigue effect was assessed by an open field test; superoxide dismutase, reactive oxygen species and malondialdehyde in skeletal muscle were determined. The mRNA levels of Akt2 and NEU in skeletal muscle were measured by real-time quantitative PCR. The activation of Akt and Nr12 was examined by western blot and immunohistofluorescence. Our results revealed that ginsenoside Rb1 significantly increased the journey and the rearing frequency, decreased the time of rest in aged rats with MSIR. In addition, ginsenoside Rb1 significantly reduced reactive oxygen species and malondialdehyde release and increased the superoxide dismutase activity of skeletal muscle in aged rats with MSIR. Ginsenoside Rb1 also increased the expression of Akt2 and NrI2 mRNA, up-regulated Akt phosphorylation and Nrf2 nuclear translocation. These findings indicate that ginsenoside Rb1 has an anti-fatigue effect on postoperative fatigue syndrome in aged rat, and the mechanism possibly involves activation of the PI3K/Akt pathway with subsequent Ni42 nuclear translocation and induction of antioxidant enzymes. (C) 2014 Elsevier B.V. All rights reserved.