Inhibitors of protein kinases and phosphatases alter root morphology and disorganize cortical microtubules
Inhibitors of protein kinases and phosphatases alter root morphology and disorganize cortical microtubules
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DOI：
10.1104/pp.113.2.493
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发表时间：
1997-02-01
期刊：
影响因子：
7.4
通讯作者：
Wilson, JE
中科院分区：
文献类型：
--
作者：
Baskin, TI;Wilson, JE
为了研究控制植物形态发生的分子机制，我们研究了拟南芥主根的形态和皮层微管的组织响应丝氨酸/苏氨酸蛋白磷酸酶和激酶抑制剂。我们发现，1型和2A型蛋白磷酸酶的抑制剂藜芦定，如先前报道的冈田酸和calyculin A（R.D.史密斯，J.E.威尔逊，J.C. T. I.步行者巴斯金[1994] Planta 193：516-524），抑制伸长并刺激径向扩张。测试的蛋白激酶抑制剂，白屈菜红碱，6-二甲基氨基嘌呤，H-89，K252 a，ML-9，和staurosporine都抑制伸长，但只有staurosporine明显刺激径向扩张。为了确定根肿胀的基础，我们检查了嵌入丁基甲基丙烯酸甲酯的材料的半薄切片中的皮质微管。白屈菜红碱和100 nM冈田酸，抑制伸长而不引起肿胀，没有改变皮质阵列的外观，但calyculin A，矢车菊苷，和staurosporine，引起肿胀，混乱的皮质微管。异常阵列中微管的稳定性与对照阵列中的稳定性没有明显差异，这是通过对冷或安磺灵解聚的相似敏感性来判断的。这些结果确定蛋白磷酸化和去磷酸化的要求，在一个或多个步骤，组织皮质阵列的微管。
To investigate molecular mechanisms controlling plant morphogenesis, we examined the morphology of primary roots of Arabidopsis thaliana and the organization of cortical microtubules in response to inhibitors of serine/threonine protein phosphatases and kinases. We found that cantharidin, an inhibitor of types 1 and 2A protein phosphatases, as previously reported for okadaic acid and calyculin A (R.D. Smith, J.E. Wilson, J.C. Walker, T.I. Baskin [1994] Planta 193: 516-524), inhibited elongation and stimulated radial expansion. Of the protein kinase inhibitors tested, chelerythrine, 6-dimethylaminopurine, H-89, K252a, ML-9, and staurosporine all inhibited elongation, but only staurosporine appreciably stimulated radial expansion. To determine the basis for the root swelling, we examined cortical microtubules in semithin sections of material embedded in butyl-methyl-methacrylate. Chelerythrine and 100 nM okadaic acid, which inhibited elongation without causing swelling, did not change the appearance of cortical arrays, but calyculin A, cantharidin, and staurosporine, which caused swelling, disorganized cortical microtubules. The stability of the microtubules in the aberrant arrays was not detectably different from those in control arrays, as judged by similar sensitivity to depolymerization by cold or oryzalin. These results identify protein phosphorylation and dephosphorylation as requirements in one or more steps that organize the cortical array of microtubules.