Skin microbiome and acne vulgaris: Staphylococcus, a new actor in acne
Skin microbiome and acne vulgaris: Staphylococcus, a new actor in acne
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DOI：
10.1111/exd.13296
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发表时间：
2017-09-01
影响因子：
3.6
通讯作者：
Seite, Sophie
中科院分区：
文献类型：
--
作者：
Dreno, Brigitte;Martin, Richard;Seite, Sophie
痤疮丙酸杆菌（P.acnes）、皮脂腺和毛囊角质形成细胞被认为是参与痤疮发展的三个因素。这项探索性研究调查了痤疮受试者的皮肤微生物群特征，并确定了使用 4% 红霉素或皮肤化妆品 28 天后微生物群的变化。根据 GEA 分级，使用拭子在无菌条件下从轻度至中度痤疮受试者的粉刺、丘疹脓疱性病变和非病变皮肤区域收集皮肤微生物群。使用针对细菌 16S rRNA 基因一部分的高通量测序方法对样品进行表征。总体而言，26 名受试者的微生物样本显示变形菌门和厚壁菌门过多，而放线菌门代表性不足。粉刺、丘疹和脓疱表面的葡萄球菌含量比非病变皮肤更丰富（分别为 P=.004 和 P=.003）。它们的比例随着痤疮严重程度的增加而显着增加（P
Propionibacterium acnes (P. acnes), the sebaceous gland and follicular keratinocytes are considered the three actors involved in the development of acne. This exploratory study investigated the characteristics of the skin microbiota in subjects with acne and determined microbiota changes after 28 days of application of erythromycin 4% or a dermocosmetic. Skin microbiota were collected under axenic conditions from comedones, papulo-pustular lesions and non-lesional skin areas from subjects with mild to moderate acne according to the GEA grading using swabs. Samples were characterized using a high-throughput sequencing approach that targets a portion of the bacterial 16S rRNA gene. Overall, microbiota samples from 26 subjects showed an overabundance of Proteobacteria and Firmicutes and an under-representation of Actinobacteria. Staphylococci were more abundant on the surface of comedones, papules and pustules (P=.004 and P=.003 respectively) than on non-lesional skin. Their proportions increased significantly with acne severity (P