A monoclonal antibody to human transitional‐cell carcinoma of the bladder cross‐reacting with a differentiation antigen of neutrophilic lineage
A monoclonal antibody to human transitional‐cell carcinoma of the bladder cross‐reacting with a differentiation antigen of neutrophilic lineage
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人膀胱移行细胞癌单克隆抗体与中性粒细胞系分化抗原发生交叉反应
DOI：
10.1002/ijc.2910350614
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发表时间：
1985
影响因子：
6.4
通讯作者：
G. Pizza
中科院分区：
文献类型：
--
作者：
O. Baricordi;A. Sensi;C. Vinci;L. Melchiorri;G. Fabris;E. Marchetti;F. Corrado;P. Mattiuz;G. Pizza
通过用福尔马林固定的 TCCB 细胞免疫 BALB/c 小鼠，然后将脾细胞与 SP2-OAg14 骨髓瘤系融合，获得针对人膀胱移行细胞癌 (TCCB) 的单克隆抗体 (MAb)。通过间接免疫荧光 (IIF) 选择 GF 26.7.3 MAb 作为与靶细胞的反应剂，并且对自体淋巴细胞和 Epstein-Barr 病毒 (EBV) 转化的淋巴母细胞系呈阴性。当通过 IIF 和免疫过氧化物酶技术检查时，GF 26.7.3 与高比例的膀胱癌和结肠癌发生反应，并与中性粒细胞谱系上表达的决定簇发生交叉反应。对健康受试者和白血病患者的骨髓和外周血 (PB) 以及白血病细胞系进行的 IIF 分析表明，GF 26.7.3 检测到的结构的表达仅限于中性粒细胞谱系，并且首先在早幼粒细胞水平表达。对来自靶细胞的 125 I 标记膜蛋白进行免疫沉淀和 SDS 聚丙烯酰胺凝胶电泳 (PAGE)，但放射自显影未检测到条带。此外，链霉蛋白酶不敏感性和高碘酸盐敏感性表明碳水化合物决定簇可能参与其中。
A monoclonal antibody (MAb) to human transitional‐cell carcinoma of the bladder (TCCB) was obtained by immunization of a BALB/c mouse with formalin‐fixed TCCB cells and subsequent fusion of the spleen cells with SP2‐OAg14 myeloma line. GF 26.7.3 MAb was selected by indirect immunofluorescence (IIF) as reacting agent with target cells and negative with autologous lymphocytes and Epstein‐Barr virus (EBV)‐transformed lymphoblastoid cell‐line. GF 26.7.3 reacts with a high percentage of bladder and colon carcinomas when examined by IIF and immunoperoxidase techniques and cross‐reacts with a determinant expressed on neutrophilic cell lineage. The IIF analysis performed on bone marrow and peripheral blood (PB) from healthy subjects and leukemic patients and on leukemic cell lines showed that the expression of the structure detected by GF 26.7.3 is restricted to the neutrophilic cell lineage and first expressed at the promyelocytic level. Immunoprecipitation and SDS‐polyacrylamide gel electrophoresis (PAGE) of 125 I‐labelled membrane proteins from target cells were performed, but no bands were detected by autoradiography. In addition, pronase insensitivity and periodate sensitivity suggest the possible involvement of a carbohydrate determinant.
通过鼠单克隆抗体鉴定人粒细胞表面分子。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Skubitz,KM;Pessano,S;Bottero,L;Ferrero,D;Rovera,G;August,JT
通讯作者：
August,JT
沙眼衣原体单克隆抗体：抗体特异性和抗原表征。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Stephens,RS;Tam,MR;Kuo,CC;Nowinski,RC
通讯作者：
Nowinski,RC
白血病和淋巴瘤细胞的表面标记：最新进展。
影响因子：
20.3
作者：
Foon,KA;Schroff,RW;Gale,RP
通讯作者：
Gale,RP