Interaction of Salmonella enterica with basil and other salad leaves
Interaction of Salmonella enterica with basil and other salad leaves
复制标题
DOI：
10.1038/ismej.2008.95
复制
发表时间：
2009-02-01
期刊：
影响因子：
11
通讯作者：
Frankel, Gad
中科院分区：
文献类型：
--
作者：
Berger, Cedric N.;Shaw, Robert K.;Frankel, Gad
受污染的沙拉叶已成为肠道病原体向人类传播的重要载体。英国最近爆发的肠沙门氏菌森夫滕贝格血清型（S. Senftenberg）可追溯到食用受污染的罗勒。利用 S. Senftenberg 的爆发菌株，我们发现它与罗勒、生菜、芝麻菜和菠菜的叶子结合，显示出弥漫性粘附模式。鞭毛将 S. Senftenberg 与叶表皮连接起来，而 fliC（编码 1 相鞭毛）的缺失导致粘附水平显着降低。相比之下，虽然将鼠伤寒沙门氏菌与罗勒叶表皮连接的鞭毛很普遍，但 fliC 的缺失并不影响叶子的附着水平。这些结果暗示了鞭毛在沙门氏菌叶片附着中的作用，并表明不同的沙门氏菌血清型使用菌株特异性机制附着在沙拉叶上。
Contaminated salad leaves have emerged as important vehicles for the transmission of enteric pathogens to humans. A recent outbreak of Salmonella enterica serovar Senftenberg (S. Senftenberg) in the United Kingdom has been traced to the consumption of contaminated basil. Using the outbreak strain of S. Senftenberg, we found that it binds to basil, lettuce, rocket and spinach leaves showing a pattern of diffuse adhesion. Flagella were seen linking S. Senftenberg to the leaf epidermis, and the deletion of fliC (encoding phase-1 flagella) resulted in a significantly reduced level of adhesion. In contrast, although flagella linking S. enterica serovar Typhimurium to the basil leaf epidermis were widespread, deletion of fliC did not affect leaf attachment levels. These results implicate the role of flagella in Salmonella leaf attachment and suggest that different Salmonella serovars use strain-specific mechanisms to attach to salad leaves.