Naked Selenium Nanoparticles for Antibacterial and Anticancer Treatments
Naked Selenium Nanoparticles for Antibacterial and Anticancer Treatments
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DOI：
10.1021/acsomega.9b03172
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发表时间：
2020-02-18
期刊：
影响因子：
4.1
通讯作者：
Guisbiers, Gregory
中科院分区：
文献类型：
--
作者：
Geoffrion, Luke D.;Hesabizadeh, Tina;Guisbiers, Gregory
目前，抗生素耐药性和癌症是两个最重要的公共卫生问题，每年造成超过150万人死亡，这表明抗生素和目前的化疗药物不如过去有效。纳米技术在这里作为一个潜在的解决方案。然而，目前传统的物理化学合成纳米材料的协议并不是没有环境和社会的缺点，往往涉及使用有毒催化剂。本文介绍了一种新的绿色工艺--液体中脉冲激光烧蚀（PLAL）制备纯裸硒纳米颗粒（SeNPs）。在第一组照射之后，进行另一组照射以将尺寸减小到100 nm以下，这产生具有两个主要群体的球形SeNP的胶体溶液，所述两个主要群体具有大约类似于80 nm和类似于10 nm的尺寸。第二组照射后的颗粒也显示出更高的胶体稳定性。SeNPs在0.05至25 ppm的浓度范围内对革兰氏阴性和革兰氏阳性细菌的标准和耐药性表型均显示出剂量依赖性抗菌作用。此外，SeNP在与人皮肤成纤维细胞一起培养时在高达1 ppm的浓度范围内显示出低细胞毒性作用，而在相同浓度范围内对人黑素瘤和胶质母细胞瘤细胞显示出抗癌作用。因此，本文介绍了使用完全裸露的SeNPs合成的一个新的PLAL协议作为一种新型的和有效的纳米粒子的制造工艺用于生物医学应用的可能性。
Currently, antibiotic resistance and cancer are two of the most important public health problems killing more than similar to 1.5 million people annually, showing that antibiotics and current chemotherapeutics are not as effective as they were in the past. Nanotechnology is presented here as a potential solution. However, current protocols for the traditional physicochemical synthesis of nanomaterials are not free of environmental and social drawbacks, often involving the use of toxic catalysts. This article shows the production of pure naked selenium nanoparticles (SeNPs) by a novel green process called pulsed laser ablation in liquids (PLAL). After the first set of irradiations, another set was performed to reduce the size below 100 nm, which resulted in a colloidal solution of spherical SeNPs with two main populations having sizes around similar to 80 and similar to 10 nm. The particles after the second set of irradiations also showed higher colloidal stability. SeNPs showed a dose-dependent antibacterial effect toward both standard and antibiotic-resistant phenotypes of Gram-negative and Gram-positive bacteria at a range of concentrations between 0.05 and 25 ppm. Besides, the SeNPs showed a low cytotoxic effect when cultured with human dermal fibroblasts cells at a range of concentrations up to 1 ppm while showing an anticancer effect toward human melanoma and glioblastoma cells at the same concentration range. This article therefore introduces the possibility of using totally naked SeNPs synthesized by a new PLAL protocol as a novel and efficient nanoparticle fabrication process for biomedical applications.