MR imaging of phagocytosis in experimental gliomas.
MR imaging of phagocytosis in experimental gliomas.
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DOI：
10.1148/radiology.197.2.7480707
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发表时间：
1995-11
期刊：
影响因子：
19.7
通讯作者：
C. Zimmer;R. Weissleder;K. Poss;Anna Bogdanova;S. C. Wright;W. S. Enochs
中科院分区：
文献类型：
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作者：
C. Zimmer;R. Weissleder;K. Poss;Anna Bogdanova;S. C. Wright;W. S. Enochs
目的观察胶质瘤细胞体内是否存在吞噬现象。材料与方法对13只大鼠C6胶质瘤细胞进行培养和脑内植入研究。单晶氧化铁纳米颗粒（MION）是一种吞噬模型标志物，以每公斤2-20毫克的铁剂量静脉注射给荷瘤大鼠。在多个时间点进行磁共振（MR）成像。结果：培养的胶质瘤细胞显示每10(6)个细胞摄取高达10 ng铁的MION，相当于每个细胞约50,000个颗粒。荧光标记的MION主要位于管状溶酶体中。脑内胶质瘤在MR成像上表现出特征性的信号强度变化，在给药后12小时达到峰值，持续5天；这些变化对应于肿瘤细胞对MION的摄取和随后的生物降解。结论氧化铁增强磁共振成像可以检测胶质瘤细胞的吞噬作用，并可以准确描绘肿瘤边缘。
PURPOSE To determine whether phagocytosis can be observed in vivo in glioma cells. MATERIALS AND METHODS Rat C6 glioma cells were studied in culture and after intracerebral implantation into 13 rats. Monocrystalline iron oxide nanoparticles (MION), a model marker of phagocytosis, was administered intravenously to tumor-bearing rats at 2-20 mg of iron per kilogram. Magnetic resonance (MR) imaging was performed at multiple time points. RESULTS Glioma cells in culture showed uptake of MION in amounts of up to 10 ng of iron per 10(6) cells, corresponding to approximately 50,000 particles per cell. Fluorescently labeled MION was found to be located primarily in tubular lysosomes. Intracerebral gliomas showed characteristic changes in signal intensity at MR imaging that peaked 12 hours after administration of MION and lasted up to 5 days; these changes corresponded to uptake and subsequent biodegradation of MION by tumor cells. CONCLUSION Phagocytosis of glioma cells can be detected in vivo with iron oxide-enhanced MR imaging, and this may permit accurate delineation of tumor margins.