Cloning and characterization of human estrogen receptor β isoforms
Cloning and characterization of human estrogen receptor β isoforms
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DOI：
10.1006/bbrc.1998.8738
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发表时间：
1998-06-09
影响因子：
3.1
通讯作者：
Willson, TM
中科院分区：
文献类型：
--
作者：
Moore, JT;McKee, DD;Willson, TM
由人雌激素受体β (ER β)基因产生的多个转录本已被表征。在睾丸cDNA文库中鉴定出hER β基因的三个全长同工异构体，命名为hER β 1-3。另外，从睾丸cDNA和MDA-MB 435细胞系中通过PCR扩增鉴定出hER β 4和hER β 5两个亚型。hER β 1对应于之前描述的hER β。所有五种同工异构体在hER β的配体结合域的预测螺旋10的共同位置上出现分歧，核苷酸序列与不同的外显子使用一致。通过RT-PCR分析，hER β亚型mrna在人体组织和肿瘤细胞系中表现出不同的表达模式。通过体外带移研究进一步表征了三个全长异构体hER β 1-3，表明这些异构体彼此之间以及与ER α亚型之间能够形成dna结合的同二聚体和异二聚体。(C) 1998学术出版社。
Multiple transcripts which arise from the human estrogen receptor beta (ER beta) gene have been characterized. Three full length isoforms of the hER beta gene, designated hER beta 1-3, were identified in a testis cDNA library. An additional two isoforms, designated hER beta 4 and hER beta 5, were identified by PCR amplification from testis cDNA and from the MDA-MB 435 cell line. hER beta 1 corresponds to the previously described hER beta. All five isoforms diverge at a common position within the predicted helix 10 of the ligand binding domain of hER beta, with nucleotide sequences consistent with differential exon usage. The hER beta isoform mRNAs displayed a differential pattern of expression in human tissues and in tumor cell lines when analyzed by RT-PCR. Further characterization of the three full length isoforms, hER beta 1-3, by in vitro band shift studies indicated that the isoforms were able to form DNA-binding homodimers and heterodimers with each other and with the ER alpha subtype. (C) 1998 Academic Press.