PROGESTERONE INHIBITS THE ESTROGEN-INDUCED EXPRESSION OF C-FOS MESSENGER-RIBONUCLEIC-ACID IN THE UTERUS
PROGESTERONE INHIBITS THE ESTROGEN-INDUCED EXPRESSION OF C-FOS MESSENGER-RIBONUCLEIC-ACID IN THE UTERUS
复制标题
DOI：
10.1210/en.130.6.3223
复制
发表时间：
1992-06-01
期刊：
影响因子：
4.8
通讯作者：
STANCEL, GM
中科院分区：
文献类型：
--
作者：
KIRKLAND, JL;MURTHY, L;STANCEL, GM
子宫内c-fos mRNA表达水平在3 h内达到最大值。孕激素的管理拮抗这种雌激素诱导的大鼠和小鼠的原癌基因转录水平的增加。孕酮的抑制作用在激素处理后1 h内观察到，并持续9-18 h。在大鼠中，在0.25 mg孕酮剂量下可以观察到这种效果，并且在2.5 mg剂量下最大。雌激素给药后，糖皮质激素地塞米松产生类似的抑制fos mRNA水平，但雄激素或盐皮质激素则没有。孕酮不能阻断雌二醇对c-jun或c-myc mRNA的诱导。用佛波醇酯佛波12-肉豆蔻酸酯13-乙酸酯处理后观察到的c-fos mRNA的子宫水平不降低3小时的孕酮预处理。在我们的实验条件下，孕酮不会降低雌二醇给药后子宫内雌激素受体的占用水平。这些发现与孕激素在c-fos基因水平抑制雌激素受体转录激活的机制一致。
Estradiol produces a large increase in the uterine level of c-fos mRNA, which is maximum in 3 h. The administration of progesterone antagonizes this estrogen-induced increase in protooncogene transcript levels in both the rat and mouse. The inhibitory effect of progesterone is observed within 1 h after hormone treatment and persists for 9-18 h. In the rat, this effect can be observed at a dose of 0.25 mg progesterone and is maximum at a dose of 2.5 mg. A similar inhibition of fos mRNA levels after estrogen administration is produced by the glucocorticoid dexamethasone, but not by androgens or mineralocorticoids. Progesterone does not block the induction of c-jun or c-myc mRNA by estradiol. Uterine levels of c-fos mRNA observed after treatment with the phorbol ester phorbol 12-myristate 13-acetate are not decreased by a 3-h pretreatment with progesterone. Under the conditions of our experiments, progesterone does not decrease occupied levels of nuclear estrogen receptors in the uterus after estradiol administration. These findings are consistent with a mechanism in which progesterone inhibits transcriptional activation by the estrogen receptor at the level of the c-fos gene.