The suffulta mutation in tomato reveals a novel method of plastid replication during fruit ripening
The suffulta mutation in tomato reveals a novel method of plastid replication during fruit ripening
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DOI：
10.1093/jxb/erj144
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发表时间：
2006-06-01
影响因子：
6.9
通讯作者：
Pyke, Kevin A.
中科院分区：
文献类型：
--
作者：
Forth, Daniel;Pyke, Kevin A.
番茄suffulta基因座的突变等位基因显著影响整个植株的质体分裂模式，导致叶和干细胞中的叶绿体很少，大大扩大。Suffulta植物的生长受到损害，并且具有明显苍白的茎。发育中的绿色番茄果实通常比野生型更苍白，但成熟的红色果实在颜色和色素含量上更相似。通过使用质体靶向的绿色荧光蛋白，在成熟suffulta果实的基本质体表型揭示，扩大含叶绿素的叶绿体退化，并产生一个野生型样群体的成熟果实中的质体出芽和碎片化的过程中，在一个异质群体的质体衍生的结构，最终成为质体。在气孔保卫细胞中，也观察到缺乏叶绿素但含有GFP的质体衍生结构，特别是在完全缺乏正常叶绿体的保卫细胞中。如何在suffulta这种新的“复制”过程涉及到传统的质体分裂和子实体的形成进行了讨论。
Mutant alleles at the suffulta locus of tomato dramatically affect the pattern of plastid division throughout the plant, resulting in few, greatly enlarged chloroplasts in leaf and stem cells. suffulta plants are compromised in growth and have distinctly pale stems. The green developing tomato fruit are generally paler compared with the wild type, but ripe red fruit are much more similar in colour and pigment content. By using plastid-targeted green fluorescent protein, the underlying plastid phenotypes in the ripening suffulta fruit reveal that enlarged chlorophyll-containing chloroplasts degenerate and give rise to a wild type-like population of chromoplasts in ripe fruit by a process of plastid budding and fragmentation, resulting in a heterogeneous population of plastid-derived structures which eventually become chromoplasts. In stomatal guard cells, plastid-derived structures lacking chlorophyll, but containing GFP, are also observed, especially in guard cells which completely lack normal chloroplasts. How this novel 'replication' process in suffulta relates to conventional plastid division and stromule formation is discussed.