Microfilament Dynamics is Required for Root Growth under Alkaline Stress in Arabidopsis
Microfilament Dynamics is Required for Root Growth under Alkaline Stress in Arabidopsis
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DOI：
10.1111/j.1744-7909.2010.00981.x
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发表时间：
2010-11-01
影响因子：
11.4
通讯作者：
Guo, Yan
中科院分区：
文献类型：
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作者：
Zhou, Yue;Yang, Zijun;Guo, Yan
微丝(Mf)细胞骨架在植物发育中具有重要作用。最近的研究揭示了MFS在不同的应激反应中的作用。碱胁迫对植物的生长是有害的，但MFS是否在碱胁迫中起作用尚不清楚。在本研究中，我们发现，用马兜铃素B(Lat B)阻断MF组装会抑制植物的根生长，而在正常情况下，用鬼臼草素稳定MFS对植物根的生长没有显著影响。在较高的外部pH条件下，MF解聚被诱导，这与根生长的减少有关；鬼臼草苷处理部分挽救了这种减少。此外，Lat B处理进一步降低了在高pH条件下生长的幼苗的存活率。然而，较高的外部pH(8.0)并不影响微球的体外稳定性。综上所述，我们的结果表明，碱胁迫可能触发了一种信号，该信号导致了MFS的动态，进而调节了根的生长。
The microfilament (MF) cytoskeleton has crucial functions in plant development. Recent studies have revealed the function of MFs in diverse stress response. Alkaline stress is harmful to plant growth; however, it remains unclear whether the MFs play a role in alkaline stress. In the present study, we find that blocking MF assembly with latrunculin B (Lat B) leads to inhibition of plant root growth, and stabilization of MFs with phalloidin does not significantly affect plant root growth under normal conditions. In high external pH conditions, MF de-polymerization is induced and that associates with the reduction of root growth; phalloidin treatment partially rescues this reduction. Moreover, Lat B treatment further decreases the survival rate of seedlings growing in high external pH conditions. However, a high external pH (8.0) does not affect MF stability in vitro. Taken together, our results suggest that alkaline stress may trigger a signal that leads the dynamics of MFs and in turn regulates root growth.