The RSC nucleosome-remodeling complex is required for Cohesin's association with chromosome arms
The RSC nucleosome-remodeling complex is required for Cohesin's association with chromosome arms
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DOI：
10.1016/s1097-2765(04)00103-0
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发表时间：
2004-03-12
期刊：
影响因子：
16
通讯作者：
Laurent, BC
中科院分区：
文献类型：
--
作者：
Huang, J;Hsu, JM;Laurent, BC
染色体分离的保真度要求粘连蛋白复合物在着丝粒和沿染色体臂的离散位点将新复制的姐妹染色单体结合在一起。酵母 2 mu 质粒的分离还需要粘连蛋白，该粘连蛋白被募集到质粒分割位点。在此，我们报道 RSC 染色质重塑复合物调节粘连蛋白与着丝粒和染色体臂的差异关联。 RSC 在中央粘连蛋白亚基 Mcd1p 之前 15 分钟，以细胞周期调节的方式在染色体臂和质粒粘连蛋白结合位点上循环。我们发现，在 rsc 突变体中，Mcd1 p 无法与染色体臂结合，但仍与着丝粒结合，因此，有丝分裂姐妹染色体的臂区提前分离，而着丝粒的内聚力不受影响。我们的数据表明 RSC 在促进粘连蛋白特异性加载到染色体臂上的作用，从而确保姐妹染色单体的粘聚力和正确的染色体分离。
The fidelity of chromosome segregation requires that the cohesin protein complex bind together newly replicated sister chromatids both at centromeres and at discrete sites along chromosome arms. Segregation of the yeast 2 mu plasmid also requires cohesin, which is recruited to the plasmid partitioning locus. Here we report that the RSC chromatin-remodeling complex regulates the differential association of cohesin with centromeres and chromosome arms. RSC cycles on and off chromosomal arm and plasmid cohesin binding sites in a cell cycle-regulated manner 15 min preceding Mcd1p, the central cohesin subunit. We show that in rsc mutants Mcd1 p fails to associate with chromosome arms but still binds to centromeres, and that consequently, the arm regions of mitotic sister chromosomes separate precociously while cohesion at centromeres is unaffected. Our data suggest a role for RSC in facilitating the loading of cohesin specifically onto chromosome arms, thereby ensuring sister chromatid cohesion and proper chromosome segregation.