Glutathione improves survival of cryopreserved embryogenic calli of Agapanthus praecox subsp orientalis
Glutathione improves survival of cryopreserved embryogenic calli of Agapanthus praecox subsp orientalis
复制标题
谷胱甘肽提高了百子莲东方亚种冷冻保存的胚性愈伤组织的存活率
DOI：
10.1007/s11738-016-2271-y
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发表时间：
2016-10-01
影响因子：
2.6
通讯作者：
Shen, Xiao-hui
中科院分区：
文献类型：
--
作者：
Chen, Guan-qun;Ren, Li;Shen, Xiao-hui
The effect of supplementation of reduced glutathione (GSH) to cryoprotectant solution on the generation of reactive oxygen species (ROS) (e.g., H2O2, OH-, and O-2(center dot-)) and antioxidants (e.g., SOD, POD, CAT, AsA, and GSH), as well as membrane lipid peroxidation (i.e., MDA content) mitigation in cryopreserving of embryogenic calli (EC) of Agapanthus praecox subsp. orientalis was investigated. The vitrification-based cryopreservation method was used in this study. The addition of GSH at a final concentration of 0.08 mM to the cryoprotectant solution has significantly improved cryotolerance of A. praecox EC. The EC post-thaw survival rate increased by 68.34 % using the cryoprotectant solution containing 0.08 mM GSH as compared to the control (GSH-free). EC treated with GSH displayed the reduction in OH- generation activity and the contents of H2O2 and MDA, as well as enhancement in the inhibition of O-2(center dot-) generation and the antioxidant activity. Treatment with exogenous GSH also increased endogenous AsA and GSH contents after dehydration step. Expression of stress-responsive genes, e.g., peroxidase (POD), peroxiredoxin, ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and glutathione peroxidase (GPX), was also increased during cryopreservation processes. The expression of DAD1 (Defender against apoptotic cell death) was elevated, while cell death-related protease SBT was suppressed. These results demonstrated that the addition of GSH to cryoprotectant solution affects the ROS level and could effectively improve survival of A. praecox EC through enhancing antioxidant enzyme activities and decreasing cell death.
