Loss-of-Function Mutations of Retromer Large Subunit Genes Suppress the Phenotype of an Arabidopsis zig Mutant That Lacks Qb-SNARE VTI11
Loss-of-Function Mutations of Retromer Large Subunit Genes Suppress the Phenotype of an Arabidopsis zig Mutant That Lacks Qb-SNARE VTI11
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DOI：
10.1105/tpc.109.069294
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发表时间：
2010-01-01
期刊：
影响因子：
11.6
通讯作者：
Morita, Miyo Terao
中科院分区：
文献类型：
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作者：
Hashiguchi, Yasuko;Niihama, Mitsuru;Morita, Miyo Terao
拟南芥zigzag（Arabidopsis thaliana zigzag）是Qb-SNARE VTI 11基因的一个功能缺失突变体，该基因参与高尔基体网络和液泡之间的膜运输。zig-1在地上部向重力性和形态学上表现出异常。在这里，我们报告，功能丧失的逆转录酶大亚基突变体部分抑制zig-1表型。此外，我们证明了拟南芥的三个旁系同源VPS 35基因在zig-1抑制方面具有部分重叠但不同的遗传功能。使用内胚层特异性SCR启动子的组织特异性互补实验显示VPS 35 B或VPS 35 C的表达不能补充VPS 35 A的功能。这些数据表明，存在功能专门的旁系同源VPS 35基因，但共享共同的功能。
Arabidopsis thaliana zigzag (zig) is a loss-of-function mutant of Qb-SNARE VTI11, which is involved in membrane trafficking between the trans-Golgi network and the vacuole. zig-1 exhibits abnormalities in shoot gravitropism and morphology. Here, we report that loss-of-function mutants of the retromer large subunit partially suppress the zig-1 phenotype. Moreover, we demonstrate that three paralogous VPS35 genes of Arabidopsis have partially overlapping but distinct genetic functions with respect to zig-1 suppression. Tissue-specific complementation experiments using an endodermis-specific SCR promoter show that expression of VPS35B or VPS35C cannot complement the function of VPS35A. The data suggest the existence of functionally specialized paralogous VPS35 genes that nevertheless share common functions.