Light is required for low-CO2-mediated induction of transcripts encoding components of the CO2-concentrating mechanism in the cyanobacterium Synechococcus elongatus: analysis by quantitative reverse transcription - polymerase chain reaction
Light is required for low-CO2-mediated induction of transcripts encoding components of the CO2-concentrating mechanism in the cyanobacterium Synechococcus elongatus: analysis by quantitative reverse transcription - polymerase chain reaction
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DOI：
10.1139/b05-055
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发表时间：
2005-07
期刊：
影响因子：
1.1
通讯作者：
P. McGinn;Meaghan J. Jones;Angela B Macdonald;D. Campbell
中科院分区：
文献类型：
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作者：
P. McGinn;Meaghan J. Jones;Angela B Macdonald;D. Campbell
在无机碳（Ci）限制的条件下，通过诱导CO2浓缩机制（CCM）来提高蓝藻的光合效率，所述CCM提高羧化体中初级羧化酶核酮糖-1，5-二磷酸羧化酶β-加氧酶（Rubisco）周围的CO2浓度。目前尚不清楚蓝藻中CCM诱导调节相关的低Ci传感的分子细节。我们已经应用了定量逆转录-聚合酶链反应技术，以监测在细长聚球藻的CCM相关的关键转录本的丰度在各种实验条件下，探测CCM诱导所需的条件的目的。尽管有初步证据表明，在黑暗中，细长聚球藻在低CI下强烈诱导cmPA，sbtA和chpY转录本，随后的实验中，在收获过程中消除了室内光的污染水平，表明光是诱导这些transc所必需的。
Photosynthetic efficiency in cyanobacteria is improved under conditions of inorganic carbon (Ci) limitation by the induction of a CO2-concentrating mechanism (CCM) that elevates the CO2 concentration around the primary carboxylase ribulose-1,5-bisphosphate carboxylaseoxygenase (Rubisco) in the carboxysome. The molecular details of low-Ci sensing in relation to regulation of CCM induction in cyanobacteria are not presently known. We have applied a quantitative reverse transcription polymerase chain reaction technique to monitor the abundance of key CCM-related transcripts in Synechococcus elongatus under a variety of experimental conditions with the aim of probing the conditions required for CCM induction. Despite preliminary evidence for strong induction of cmpA, sbtA, and chpY transcripts in the dark under low Ci in Synechococcus elongatus, subsequent experiments in which contaminating levels of room light during harvest were eliminated demonstrated that light is required for induction of these transc...