A two-entropies analysis to identify functional positions in the transmembrane region of class A G protein-coupled receptors
A two-entropies analysis to identify functional positions in the transmembrane region of class A G protein-coupled receptors
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DOI：
10.1002/prot.20899
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发表时间：
2006-06-01
影响因子：
2.9
通讯作者：
Ijzerman, Adriaan P.
中科院分区：
文献类型：
--
作者：
Ye, Kai;Lameijer, Eric-Wubbo M.;Ijzerman, Adriaan P.
G蛋白偶联受体（GPCR）跨膜区的残基对于配体结合和激活是重要的，但对单个位置的功能知之甚少。使用A类GPCR（分组在亚家族）的序列比对，我们提出了一个所谓的“两熵分析”，以确定在跨膜区域的A类GPCR的各个位置的潜在作用。在我们的方法中，这些位置看起来很分散，而根据其生物学功能主要是聚集的。我们的方法出现上级相比，其他生物信息学方法，如进化轨迹法，熵变图，相关突变分析，定性和定量。Proteins 2006;63：1018-1030. (c)2006 Wiley-Liss，Inc.
Residues in the transmembrane region of G protein-coupled receptors (GPCRs) are important for ligand binding and activation, but the function of individual positions is poorly understood. Using a sequence alignment of class A GPCRs (grouped in subfamilies), we propose a so-called "two-entropies analysis" to determine the potential role of individual positions in the transmembrane region of class A GPCRs. In our approach, such positions appear scattered, while largely clustered according to their biological function. Our method appears superior when compared to other bioinformatics approaches, such as the evolutionary trace method, entropy-variability plot, and correlated mutation analysis, both qualitatively and quantitatively. Proteins 2006;63:1018-1030. (c) 2006 Wiley-Liss, Inc.