TRANS-OVARIAL TRANSMISSION OF ST-LOUIS ENCEPHALITIS-VIRUS BY CULEX-PIPIENS COMPLEX MOSQUITOS
TRANS-OVARIAL TRANSMISSION OF ST-LOUIS ENCEPHALITIS-VIRUS BY CULEX-PIPIENS COMPLEX MOSQUITOS
复制标题
DOI：
10.4269/ajtmh.1981.30.699
复制
发表时间：
1981-01-01
影响因子：
3.3
通讯作者：
BOLIN, RA
中科院分区：
文献类型：
--
作者：
FRANCY, DB;RUSH, WA;BOLIN, RA
本实验旨在确定圣路易斯脑炎（SLE）病毒在C. pipiens complex蚊子，美国中东部SLE病毒的主要载体。1978年，使用了从田纳西州孟菲斯和伊利诺斯州麦克利安斯伯勒实地收集的蚊子; 1979年期间，使用了来自伊利诺斯州芝加哥和田纳西州孟菲斯的定殖蚊子。用分离自库蚊的SLE病毒株接种病毒血症雏鸡，经蚊虫取食感染。1976年从田纳西州的孟菲斯收集的pipiens复合蚊。在1979年的实验中，将后代幼虫和成年蚊子保持在2个温度下，18 ℃。25度。C.通过鸭胚细胞培养中的空斑试验、白纹伊蚊C6/36细胞接种和免疫荧光检查来检测子代的病毒。在1978年，大多数测试的后代来自第一个卵巢周期，并记录了TOT的单一发生。1979年，46，856例第一卵巢周期子代中发生单一TOT; 9234例第二卵巢周期子代中有7例感染。孟菲斯蚊子后代的TOT率高于芝加哥蚊子，并且蚊子保持在18 ℃。C比25. C;这些差异无统计学意义。4个阳性样本池为雌性，3个样本池用雏鸡喂养以进行传播尝试。阳性的芝加哥蚊子池没有传播，但孟菲斯的两个蚊子池都成功传播了病毒。SLE病毒在第一和第二卵巢周期的后代中的总TOT率分别为1/4551和1/1460。这些结果的意义，因为它们涉及到SLE病毒的自然史进行了讨论。
Experiments were conducted to determine whether transovarial transmission (TOT) of St. Louis encephalitis (SLE) virus occurs in C. pipiens complex mosquitoes, the principal vectors of SLE virus in the central-eastern USA. In 1978, field-collected mosquitoes from Memphis, Tennessee and McLeansboro, Illinois, were used; during 1979, colonized mosquitoes from Chicago, Illinois and Memphis, Tennessee were used. Mosquitoes were infected by feeding on viremic chicks inoculated with an SLE virus strain isolated from C. pipiens complex mosquitoes collected from Memphis, Tennessee in 1976. During the 1979 experiments, progeny larval and adult mosquitoes were held at 2 temperatures, 18.degree. and 25.degree. C. Progeny were tested for virus by plaque assay in duck embryo cell cultures, inoculation of Aedes albopictus C6/36 cells and examination by immunofluorescence. In 1978, most of the progeny tested were from the first ovarian cycle and a single occurrence of TOT was documented. In 1979, a single TOT occurred from 46,856 first ovarian cycle progeny; 7 of 9234 progeny of the second ovarian cycle were infected. The rate of TOT was higher for progeny of Memphis than Chicago mosquitoes and for mosquitoes held at 18.degree. C than at 25.degree. C; these differences were not statistically significant. Four positive pools were females and 3 were fed on chicks for transmission attempts. The positive Chicago mosquito pool failed to transmit but both Memphis pools successfully transmitted virus. The overall rates of TOT of SLE virus in progeny of the first and second ovarian cycle were, respectively, 1/4551 and 1/1460. The significance of these results as they relate to the natural history of SLE virus is discussed.