Evidence for the Participation of ATP-sensitive Potassium Channels in the Antinociceptive Effect of Curcumin.
Evidence for the Participation of ATP-sensitive Potassium Channels in the Antinociceptive Effect of Curcumin.
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DOI：
10.3344/kjp.2012.25.4.221
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Castañeda-Hernández G
中科院分区：
文献类型：
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作者：
De Paz-Campos MA;Chávez-Piña AE;Ortiz MI;Castañeda-Hernández G
据报道，姜黄素是姜黄（也称为姜黄）的主要活性化合物，具有抗伤害特性。本研究的目的是检查 ATP 敏感钾通道（KATP 通道），特别是 L-精氨酸-一氧化氮-环 GMP-KATP 通道途径在姜黄素抗伤害作用中的参与。通过在 Wistar 大鼠的右后爪足底内注射 1% 福尔马林来诱发疼痛。福尔马林引起的退缩行为被解释为伤害感受的表达。在使用一氧化氮合酶抑制剂 L-NAME、可溶性鸟苷酸环化酶抑制剂 ODQ 和 KATP 通道阻断剂格列本脲进行局部预处理的情况下，探讨了口服姜黄素的镇痛作用。在 1% 福尔马林试验中，口服姜黄素产生剂量依赖性的镇痛作用。局部 L-NAME 或 ODQ 不会改变姜黄素诱导的镇痛作用，但格列本脲会显着损害姜黄素诱导的镇痛作用。我们的结果证实姜黄素是一种有效的抗伤害剂。姜黄素诱导的镇痛作用似乎涉及外周水平 KATP 通道的参与，因为局部注射格列本脲可阻止其作用。然而，KATP 通道的激活并不是通过 L-精氨酸-一氧化氮-cGMP-KATP 通道途径的激活而发生的。
It has been reported that curcumin, the main active compound of Curcuma longa, also known as turmeric, exhibits antinociceptive properties. The aim of this study was to examine the participation of ATP-sensitive potassium channels (KATP channels) and, in particular, that of the L-arginine-nitric oxide-cyclic GMP-KATP channel pathway, in the antinociceptive effect of curcumin. Pain was induced by the intraplantar injection of 1% formalin in the right hind paw of Wistar rats. Formalin-induced flinching behavior was interpreted as an expression of nociception. The antinociceptive effect of oral curcumin was explored in the presence and absence of local pretreatment with L-NAME, an inhibitor of nitric oxide synthase, ODQ, an inhibitor of soluble guanylyl cyclase, and glibenclamide, a blocker of KATP channels. Oral curcumin produced a dose-dependent antinociceptive effect in the 1% formalin test. Curcumin-induced antinociception was not altered by local L-NAME or ODQ, but was significantly impaired by glibenclamide. Our results confirm that curcumin is an effective antinociceptive agent. Curcumin-induced antinociception appears to involve the participation of KATP channels at the peripheral level, as local injection of glibenclamide prevented its effect. Activation of KATP channels, however, does not occur by activation of the L-arginine-nitric oxide-cGMP-KATP channel pathway.