Dehydrin from citrus, which confers in vitro dehydration and freezing protection activity, is constitutive and highly expressed in the flavedo of fruit but responsive to cold and water stress in leaves
Dehydrin from citrus, which confers in vitro dehydration and freezing protection activity, is constitutive and highly expressed in the flavedo of fruit but responsive to cold and water stress in leaves
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DOI：
10.1021/jf035216
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发表时间：
2004-04-07
影响因子：
6.1
通讯作者：
Zacarias, L
中科院分区：
文献类型：
--
作者：
Sanchez-Ballesta, MT;Rodrigo, MJ;Zacarias, L
从冷敏型福临门柑橘（Citrus clementina Hort. Ex Tanaka x Citrus reticulata布兰科）果皮中克隆了一个编码福临门蛋白的cDNA，命名为Crcor 15。预测的CrCOR 15蛋白是来自柑橘的密切相关的k2 S家族的成员，因为它包含两个串联重复的不寻常的柑橘K-片段和一个S-片段（丝氨酸簇）在一个不寻常的C-末端位置。Crcor 15 mRNA在果实发育和成熟过程中在果皮中持续高表达。外果皮中Crcor 15 mRNA的相对丰度估计高于总RNA的1%。高mRNA水平保持不变，在冷藏（2degreesC）和非冷藏（12 degreesC）温度下的果实贮藏期间，它被抑制的调节处理（37 degreesC下3天），诱导耐冷性。因此，Crcor 15基因的表达与柑橘果实耐冷性的获得无关。然而，Crcor 15，这是几乎没有检测到在非胁迫柑橘叶片，迅速诱导响应低温和水分胁迫。COR 15蛋白在大肠杆菌中表达，纯化的蛋白质赋予体外保护免受冷冻和脱水失活。讨论了柑橘COR 15的潜在作用。
A cDNA encoding a dehydrin was isolated from the flavedo of the chilling-sensitive Fortune mandarin fruit (Citrus clementina Hort. Ex Tanaka x Citrus reticulata Blanco) and designed as Crcor15. The predicted CrCOR15 protein is a K2S member of a closely related dehydrin family from Citrus, since it contains two tandem repeats of the unusual Citrus K-segment and one S-segment (serine cluster) at an unusual C-terminal position. Crcor15 mRNA is consistently and highly expressed in the flavedo during fruit development and maturation. The relative abundance of Crcor15 mRNA in the flavedo was estimated to be higher than 1% of total RNA. The high mRNA level remained unchanged during fruit storage at chilling (2degreesC) and nonchilling (12degreesC) temperatures, and it was depressed by a conditioning treatment (3 days at 37degreesC) that induced chilling tolerance. Therefore, the expression of Crcor15 appears not to be related to the acquisition of chilling tolerance in mandarin fruits. However, Crcor15, which was barely detected in unstressed mandarin leaves, was rapidly induced in response to both low temperature and water stress. COR15 protein was expressed in Escherichia coli, and the purified protein conferred in vitro protection against freezing and dehydration inactivation. The potential role of Citrus COR15 is discussed.