Higher allergenicity of high molecular weight hydrolysed wheat protein in cosmetics for percutaneous sensitization
Higher allergenicity of high molecular weight hydrolysed wheat protein in cosmetics for percutaneous sensitization
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DOI：
10.1111/j.1600-0536.2012.02168.x
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发表时间：
2013-02-01
影响因子：
5.5
通讯作者：
Morita, Eishin
中科院分区：
文献类型：
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作者：
Chinuki, Yuko;Takahashi, Hitoshi;Morita, Eishin
背景 小麦蛋白衍生物在全世界范围内用于多种产品。麸质在商业上按原样使用或进行修饰（例如水解），以克服其不溶性。已经描述了几起接触化妆品中的水解小麦蛋白 (HWP) 后发生接触性荨麻疹的病例，或食品或非食品中的脱酰胺麸质引起的过敏反应。目的 评价经皮致敏性较高的 HWP 类型。方法 我们招募了 7 名小麦依赖性运动引起的过敏反应患者，他们主要通过使用含有 HWP 的洗面奶经皮和/或鼻结膜途径对 HWP 敏感。通过 IgE 免疫印迹和六种 HWP 变体的嗜碱性粒细胞 CD203c 表达证实了对小麦蛋白的反应。结果所有患者的IgE均对由大多肽聚集体组成的HWP产生反应。还发现高分子量 (MW) HWP 可诱导嗜碱性粒细胞 CD203c 表达的显着增强。结论 由大多肽聚集体组成的 HWP 可能比低分子量的 HWP 诱导更大程度的致敏。嗜碱性粒细胞表面 CD203c 表达可用于评估 HWP 的过敏性。
Background Wheat protein derivatives are used in a variety of products worldwide. Gluten is commercially used as is' or with modifications such as hydrolysis, which is carried out to overcome its insolubility. Several cases of contact urticaria following exposure to hydrolysed wheat protein (HWP) in cosmetics or of anaphylaxis caused by deamidated gluten in food or non-food products have been described. Objectives To evaluate the types of HWP that have higher allergenicity for percutaneous sensitization. Methods We enrolled 7 patients with wheat-dependent exercise-induced anaphylaxis who had been sensitized to HWP primarily through the percutaneous and/or the rhinoconjunctival route by using facial soap containing HWP. Reaction to wheat proteins was confirmed by IgE immunoblotting and basophil CD203c expression with six HWP variants. Results The IgE of all the patients reacted to HWPs composed of large polypeptide aggregates. High molecular weight (MW) HWPs were also found to induce significant enhancement of basophil CD203c expression. Conclusions HWPs composed of large polypeptide aggregates possibly induce sensitization to a greater degree than lower-MW HWPs. Basophil surface CD203c expression is useful for evaluating the allergenicity of HWPs.