Characterization of Nucleoside Diphosphate Kinase from Moderately Halophilic Eubacteria
Characterization of Nucleoside Diphosphate Kinase from Moderately Halophilic Eubacteria
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中度嗜盐真细菌核苷二磷酸激酶的表征
DOI：
10.1271/bbb.65.2343
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Tokunaga
中科院分区：
文献类型：
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作者：
Yasushi Yonezawa;H. Tokunaga;M. Ishibashi;M. Tokunaga
通过ATP-琼脂糖柱层析和苯基-5PW柱层析，从自然分离的中度嗜盐真细菌中纯化了核苷二磷酸激酶，使其具有明显的均一性。飞行时间质谱仪测定该酶的相对分子质量为15 kDa。该蛋白在SDS-PAGE上表现出异常的迁移率，这是典型的嗜盐蛋白。它在从0到4.0M的盐浓度范围内都是稳定和活性的。
Nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by ATP-agarose and phenyl-5PW column chromatographies. The molecular mass of this enzyme was 15 kDa by time-of-flight mass-spectrometry. This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl.