Immunocytochemical evidence for a progesterone receptor in neurons and glial cells of the rat spinal cord
Immunocytochemical evidence for a progesterone receptor in neurons and glial cells of the rat spinal cord
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DOI：
10.1016/s0304-3940(00)01191-5
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发表时间：
2000-07-07
影响因子：
2.5
通讯作者：
De Nicola, AF
中科院分区：
文献类型：
--
作者：
Labombarda, F;Guennoun, R;De Nicola, AF
使用识别 B 型孕酮受体 (PR) 的 KC 146 单克隆抗体和免疫细胞化学技术，我们研究了大鼠脊髓中是否存在 PR 免疫反应细胞。来自腹角第九层的神经元、灰质和白质中的神经胶质细胞以及室管膜细胞均为 PR 阳性。未观察到卵巢切除大鼠中雌激素诱导 PR 的证据。通过计算机图像分析测量，脊髓神经元 PR 免疫染色强度没有显着的性别差异。在雌激素雌性大鼠的垂体和子宫中，PR 显示出严格的核定位，而在脊髓的神经元和神经胶质细胞中，PR 定位在细胞质和/或细胞核以及某些细胞突起中。该受体可能与脊髓中描述的黄体酮的一些生物效应有关。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
Using the KC 146 monoclonal antibody recognizing the B-form of the progesterone receptor (PR) and immunocytochemical techniques, we investigated if PR-immunoreactive cells are present in the rat spinal cord. Neurons from ventral horn Lamina IX, glial cells in gray and white matter and ependymal cells were PR-positive. Evidence for estrogen-inducibility of PR in ovariectomized rats was not observed. There were no significant gender differences in neuronal PR immunostaining intensity in the spinal cord, measured by computerized image ana lysis. In pituitary and uterus from estrogenized female rats, PR showed a strict nuclear localization, whereas in neurons and glial cells of the spinal cord, PR localized in cytoplasm and/or nucleus and in some cell processes. This receptor may be implicated in some of the biological effects of progesterone described in the spinal cord. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.