Japanese Huperzia serrata extract and the constituent, huperzine A, ameliorate the scopolamine-induced cognitive impairment in mice
Japanese Huperzia serrata extract and the constituent, huperzine A, ameliorate the scopolamine-induced cognitive impairment in mice
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DOI：
10.1080/09168451.2015.1052773
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
Takuya Ohba;Y. Yoshino;M. Ishisaka;N. Abe;K. Tsuruma;M. Shimazawa;M. Oyama;T. Tabira;H. Hara
中科院分区：
文献类型：
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作者：
Takuya Ohba;Y. Yoshino;M. Ishisaka;N. Abe;K. Tsuruma;M. Shimazawa;M. Oyama;T. Tabira;H. Hara
蛇足石杉是我国民间常用中药。它含有石杉碱甲，在动物模型中对记忆缺陷有保护作用;锯齿叶提取物在阿尔茨海默病（AD）模型中发挥任何作用。我们使用H。采用高效液相色谱法测定石杉碱甲的含量。测定其对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）活性的抑制作用。H.口服给予锯齿叶提取物（30 mg/kg/天）和多奈哌齐（10 mg/kg/天）7天。重复给药后，我们进行Y-迷宫和被动回避试验。H.蛇足藤提取物含0.5%石杉碱甲;锯缘叶提取物对乙酰胆碱酯酶（AChE）有抑制作用，但对BuChE无抑制作用。H.锯齿叶提取物改善小鼠的认知功能。这些结果表明，日本H.锯叶木提取物通过抑制AChE改善认知功能缺陷。因此，H.锯叶木提取物可能对预防或治疗AD中的痴呆症有价值。重复治疗蛇足石杉提取物（30毫克/公斤/天）6或7天改善东莨菪碱诱导的记忆障碍在两个行为测试。
Huperzia serrata has been used as a Chinese folk medicine for many years. It contains huperzine A, which has a protective effect against memory deficits in animal models; however, it is unclear if H. serrata extract exerts any effects in Alzheimer’s disease (AD) models. We used H. serrata collected in Japan and determined its huperzine A content using HPLC. We determined its inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity. H. serrata extract (30 mg/kg/day) and donepezil (10 mg/kg/day) were orally administrated for 7 days. After repeated administration, we performed the Y-maze and passive avoidance tests. H. serrata extract contained 0.5% huperzine A; H. serrata extract inhibited AChE, but not BuChE. H. serrata extract ameliorated cognitive function in mice. These results indicate that Japanese H. serrata extract ameliorates cognitive function deficits by inhibiting AChE. Therefore, H. serrata extract may be valuable for the prevention or treatment of dementia in AD. Repeated treatment with Huperzia serrata extract (30 mg/kg/day) for six or seven days ameliorated the memory impairment induced by scopolamine in the two behavioral tests.