Paip1 affects breast cancer cell growth and represents a novel prognostic biomarker
Paip1 affects breast cancer cell growth and represents a novel prognostic biomarker
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Paip1 影响乳腺癌细胞生长并代表一种新型预后生物标志物
DOI：
10.1016/j.humpath.2017.10.037
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发表时间：
2018-03-01
期刊：
影响因子：
3.3
通讯作者：
Lin, Zhenhua
中科院分区：
文献类型：
--
作者：
Piao, Junjie;Chen, Liyan;Lin, Zhenhua
多聚腺苷酸结合蛋白相互作用蛋白1（Paipl）调节翻译起始。越来越多的证据表明Paipl在癌症的发生和发展中起重要作用。本研究探讨Paipl在乳腺癌进展中的作用，并评估其预后价值。Paipl蛋白的细胞定位用免疫荧光法测定。采用免疫组化法检测119例乳腺癌组织和40例正常乳腺组织中Paip 1蛋白的表达。Paipl表达与乳腺癌临床病理特征之间的相关性采用卡方检验进行评估，生存曲线的差异采用对数秩检验进行分析。通过siRNA转染鉴定Paipl在乳腺癌增殖和细胞周期进展中的作用。Paip 1主要表达于癌细胞和癌组织的胞浆中。乳腺癌组织中表达阳性率为60.5%（72/119），明显高于正常乳腺组织（17.5%; 7/40）。Paipl蛋白高表达与高组织学分级、晚期临床分期和低生存率相关。多因素分析显示Paip 1是独立的预后因素。此外，通过RNAi的Paipl消耗显著降低细胞增殖并诱导细胞周期停滞。总之，我们的研究表明Paipl促进乳腺癌的生长，并可能成为预后生物标志物和治疗靶点。(C)2017爱思唯尔公司All rights reserved.
Polyadenylate-binding protein-interacting protein 1 (Paipl) regulates translational initiation. Increasing evidence suggests that Paipl plays important roles in cancer development and progression. This study explored the role of Paipl in breast cancer progression and evaluated its prognostic value. The cellular location of Paipl protein was determined using immunofluorescence. Then, Paip1 protein expression was evaluated by immunohistochemical staining in 119 breast cancers and 40 normal breast tissues. The correlation between Paipl expression and the clinicopathologic features of breast cancer was evaluated using the chi(2) test, and differences in survival curves were analyzed using log-rank tests. The role of Paipl in breast cancer proliferation and cell cycle progression was identified by siRNA transfection. Paip1 was expressed mainly in the cytoplasm of cancer cells and tissues. Expression was observed in 60.5% of the breast cancers (72/119), which was significantly higher than in normal breast tissues (17.5%; 7/40). High expression of Paipl protein was associated with high histologic grade, late clinical stage, and a low survival rate. Multivariate analysis indicated that Paip1 was an independent prognostic factor. Additionally, Paipl depletion by RNAi significantly decreased cell proliferation and induced cell cycle arrest. In conclusion, our study demonstrated that Paipl promotes the growth of breast cancers and could be a prognostic biomarker and therapeutic target. (C) 2017 Elsevier Inc. All rights reserved.