Blood oxidative stress biomarkers: Influence of sex, exercise training status, and dietary intake
Blood oxidative stress biomarkers: Influence of sex, exercise training status, and dietary intake
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DOI：
10.1016/j.genm.2008.07.002
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发表时间：
2008-09-01
期刊：
影响因子：
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通讯作者：
Fisher-Wellman, Kelsey H.
中科院分区：
文献类型：
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作者：
Bloomer, Richard J.;Fisher-Wellman, Kelsey H.
背景：众所周知，性别和生活方式因素会影响蛋白质、脂质和 DNA 的氧化。蛋白质羰基 (PC)、丙二醛 (MDA) 和 8-羟基脱氧鸟苷 (8-OHdG) 等生物标志物已常用于表征人类受试者的氧化状态。 目的：本研究比较了男性和女性之间与运动训练状态和饮食摄入相关的静息血液氧化应激生物标志物。方法：接受过运动训练和久坐的男性和女性（月经周期正常；在卵泡早期阶段报告）通过招聘传单和口碑从田纳西州孟菲斯大学、校园和周边社区招募。参与者按性别和当前运动训练状态（即受过训练或未训练）进行分类。每个人都完成了 5 天的所有食物和饮料消耗的详细食物记录。分析饮食中的千卡热量以及大量和微量营养素（维生素 C、E、A）的摄入量。在静息状态下采集静脉血样本，并分析 PC、MDA 和 8-OHdG。 结果：在 131 名参与者中（89 名男性，其中 74 名接受过运动训练，15 名未接受过训练；42 名女性，其中 22 名接受过运动训练，20 名未接受过训练；平均 [SD] 年龄为 24 141 岁），受过训练的男性和女性之间或未经训练的男性和女性之间的 PC 没有显着差异。然而，受过训练的参与者的血浆 PC（以 nmol . mg 蛋白质 (-1) 为单位测量）（平均 [SEM] 0.0966 [0.0055]）明显低于未经训练的参与者 (0.1036 [0.0098]) (P < 0.05)。与受过训练的男性 (0.6959 [0.0593]) 相比，受过训练的女性 (0.4264 [0.0559]) 的 MDA 水平（以 mu mol. L-1 为单位测量）显着较低；受过训练的男性和女性合计 (0.5621 [0.0566]) 与未经训练的男性和女性合计 (0.7397 [0.0718]) 相比；女性合计 (0.5665 [0.0611]) 与男性合计 (0.7338 [0.0789]) 相比（所有比较 P < 0.05）。任何组之间的 8-OHdG 均未发现显着差异。 PC 和 8-OHdG 均与任何饮食变量均无关，但未经训练的男性中 PC 和蛋白质百分比除外（r = 0.552；P = 0.033）。 MDA 与蛋白质摄入量呈正相关，与碳水化合物和维生素 C 摄入量的百分比呈负相关，主要是在受过训练的男性中（P
Background: Sex and lifestyle factors are known to influence the oxidation of protein, lipids, and DNA. Biomarkers such as protein carbonyls (PC), malondialdehyde (MDA), and 8-hydroxydeoxyguanosine (8-OHdG) have been commonly used in an attempt to characterize the oxidative status of human subjects.Objective: This study compared resting blood oxidative stress biomarkers, in relation to exercise training status and dietary intake, between men and women.Methods: Exercise-trained and sedentary men and women (with normal menstrual cycles; reporting during the early follicular phase) were recruited from the University of Memphis, Tennessee, campus and Surrounding community via recruitment flyers and word of mouth. Participants were categorized by sex and current exercise training status (ie, trained or untrained). Each completed a detailed 5-day food record of all food and drink consumed. Diets were analyzed for kilocalories and macro- and micronutrient (vitamins C, E, A) intake. Venous blood samples were obtained at rest and analyzed for PC, MDA, and 8-OHdG.Results: In the 131 participants (89 men, of whom 74 were exercise trained and 15 untrained, and 42 women, of whom 22 were exercise trained and 20 untrained; mean [SD] age, 24 141 years), PC did not differ significantly between trained men and women or between untrained men and women. However, trained participants had significantly lower plasma PC (measured in nmol . mg protein(-1)) (mean [SEM] 0.0966 [0.0055]) than did untrained participants (0.1036 [0.0098]) (P < 0.05). MDA levels (measured in mu mol . L-1) were significantly lower in trained women (0.4264 [0.0559]) compared with trained men (0.6959 [0.0593]); in trained men and women combined (0.5621 [0.0566]) compared with untrained men and women combined (0.7397 [0.0718]); and in women combined (0.5665 [0.0611]) compared with men combined (0.7338 [0.0789]) (P < 0.05 for all comparisons). No significant differences were noted between any groups for 8-OHdG. Neither PC nor 8-OHdG were correlated to any dietary variable, with the exception of PC and percent of protein in untrained men (r = 0.552; P = 0.033). MDA was positively correlated to protein intake and negatively correlated to percent of carbohydrate and vitamin C intake, primarily in trained men (P