Dynamic analysis of m6A methylation spectroscopy during progression and reversal of hepatic fibrosis
Dynamic analysis of m6A methylation spectroscopy during progression and reversal of hepatic fibrosis
复制标题
肝纤维化进展和逆转过程中m6A甲基化谱的动态分析
DOI：
10.2217/epi-2019-0365
复制
发表时间：
2020-11-11
期刊：
影响因子：
3.8
通讯作者：
Pan, Qiuhui
中科院分区：
文献类型：
--
作者：
Cui, Zhongqi;Huang, Nan;Pan, Qiuhui
目的：动态分析肝纤维化进展和逆转过程中m6A甲基化的差异。材料与方法：通过腹腔注射CCl4诱导C57/BL6小鼠肝纤维化。连续注射CCl4后停药，建立肝纤维化逆转模型。动态m6A甲基化在不同阶段肝纤维化的进展和逆转中使用MeRIP-Seq进行评估。结果：在肝纤维化过程中，m6A差异甲基化主要富集于氧化应激和细胞色素代谢相关过程，而m6A差异甲基化主要富集于肝纤维化逆转过程中免疫应答和细胞凋亡相关过程。结论：m6A甲基化在肝纤维化的进展和逆转中起重要作用。
Aim: To dynamically analyze the differential m6A methylation during the progression and reversal of hepatic fibrosis. Materials & methods: We induced hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection of CCl4. Dynamic m6A methylation was evaluated using MeRIP-Seq in the progression and reversal of hepatic fibrosis at different stages. Result: During the hepatic fibrosis, differential m6A methylation was mainly enriched in processes associated with oxidative stress and cytochrome metabolism, while differential m6A methylation was mainly enriched in processes associated with immune response and apoptosis in the hepatic fibrosis reversal. Conclusion: m6A methylation plays an important role in the progression and reversal of hepatic fibrosis.