Clinicopathologic and Immunohistochemical Correlates of CTNNB1 Mutated Endometrial Endometrioid Carcinoma
Clinicopathologic and Immunohistochemical Correlates of CTNNB1 Mutated Endometrial Endometrioid Carcinoma
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DOI：
10.1097/pgp.0000000000000583
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发表时间：
2020-03-01
影响因子：
2.4
通讯作者：
Howitt, Brooke E.
中科院分区：
文献类型：
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作者：
Costigan, Danielle C.;Dong, Fei;Howitt, Brooke E.
在低级别低分期疾病患者中，具有外显子3 CTNNB 1突变的子宫内膜类腺癌（EEC）是一种更具侵袭性的肿瘤亚群。因此，前瞻性识别这些病例可能具有临床意义。本研究的目的是探讨β-连环蛋白和细胞周期蛋白D1免疫组化的可行性，以确定携带CTNNB 1突变的内皮细胞，并评估与外显子3 CTNNB 1突变的内皮细胞的临床病理特征。使用靶向下一代测序板从先前测序的子宫内膜癌队列中鉴定出39个CTNNB 1突变的EEC和40个CTNNB 1野生型EEC。对所有病例进行β-连环蛋白和细胞周期蛋白D1的免疫组化。免疫组化结果与CTNNB 1突变状态和临床病理参数相关。CTNNB 1突变型患者比CTNNB 1野生型患者更年轻（56.2 vs. 61.5岁; P=0.033）。β-连环蛋白表达与外显子3 CTNNB 1突变相关（P
Endometrial endometrioid carcinomas (EECs) with exon 3 CTNNB1 mutations characterize a more aggressive subset of tumors in patients with low-grade low-stage disease. Thus, prospectively identifying these cases may be clinically relevant. The aim of this study was to examine the feasibility of beta-catenin and Cyclin D1 immunohistochemistry to identify EECs harboring CTNNB1 mutations and to evaluate the clinicopathologic features of EECs with exon 3 CTNNB1 mutations. Thirty-nine CTNNB1 mutated EECs and 40 CTNNB1 wild-type EECs were identified from a cohort of previously sequenced endometrial carcinomas using a targeted next-generation sequencing panel. Immunohistochemistry for beta-catenin and Cyclin D1 was performed on all cases. Immunohistochemistry results were correlated with CTNNB1 mutation status and clinicopathologic parameters. Patients with CTNNB1 mutated EECs were younger than those with CTNNB1 wild-type (56.2 vs. 61.5 y; P=0.033). Nuclear beta-catenin expression correlated with exon 3 CTNNB1 mutation (P